Protocols

Standardized protocol for comparing anesthetic effectiveness by measuring induction and recovery times in salamanders using righting reflex as the endpoint. Animals are immersed in anesthetic solution...

Systematic compilation of demographic datasets from multiple species across different taxonomic groups and life histories to enable comparative analysis of population sensitivity to environmental vari...

Systematic comparison of first-order kinetic vs. dormancy-based models for predicting soil CO2 respiration under varying moisture conditions using multi-scale experimental datasets.

Development of graphical models and mathematical frameworks to categorize and analyze visitor-mediated trade-offs in floral evolution. Includes classification of phenotypic and opportunity trade-offs ...

Construction and testing of controlled humidity chambers using saturated salt solutions to maintain precise relative humidity conditions for validating biophysical models against live amphibians

Bumble bee competition levels are experimentally manipulated in controlled flight cage environments by varying the number of conspecific and heterospecific co-visitors while recording focal bee foragi...

In situ freezing treatment using liquid nitrogen and heat exchanger to gradually cool plant tissues to -30°C. Designed to simulate natural freezing events while allowing controlled temperature ramping...

A factorial greenhouse experiment testing effects of 3°C warming and species interactions on biomass production of a hemiparasitic plant and its grass host. Plants were grown in ambient vs. warmed gre...

Neighbor vegetation was planted around potted B. stricta plants at controlled densities (0, 25, 50, 75, 100%) within a 5 cm radius to test causal relationships between neighbor density and plant fitne...

Laboratory-controlled temperature treatments using paired experimental boxes in contrasting thermal environments to test effects of temperature on insect development rates and voltinism.

Controlled experiment testing effects of different soil treatments on transplant survival, growth, and reproductive success with daily watering and weekly monitoring.

Collection of soot particles from controlled fires using dead plant material in fume hood setup. Various collection surfaces positioned at different heights above fire with air duct directing smoke fl...

Integrated approach combining NEON AOP imaging spectroscopy with synchronized field collection of foliar samples for laboratory analysis and trait measurement. Designed to enable landscape-scale trait...

Depth profile sampling and analysis to constrain erosion rates and inheritance using cosmogenic ¹⁰Be concentrations at different depths below surface. Uses Monte Carlo modeling to determine exposure a...

Standard method for determining exposure ages of rock surfaces using in-situ produced cosmogenic ¹⁰Be nuclides measured by accelerator mass spectrometry. Involves sample collection, quartz purificatio...

A comprehensive method for calculating multiple water productivity measures at the county scale using crop marketing revenues, production costs, and consumptive water use data to assess economic retur...

Fish captured by sport anglers during creel surveys are measured for weight and length, then analyzed using improved weight-length relationships and relative weight calculations to assess fish conditi...

Laboratory assays measuring critical thermal minimum (CTmin) and chill coma recovery time (CCRT) to quantify cold tolerance in Drosophila populations.

Sequential exposure of plants to different natural enemies to test for cross-resistance effects mediated by plant defense pathways, measuring performance of second attacker.

Mathematical framework deriving power-law relationships between pCO2 and equilibrium solute concentrations based on reaction stoichiometry and equilibrium constants. Applied to assess silicate weather...

Cryo-TEM analysis of colloidal particles involving vitrification of samples on holey carbon grids followed by high-resolution imaging at cryogenic temperatures to preserve native morphology.

Calculation of early life adversity by summing nine binary adversity measures experienced from emergence to sexual maturity, with adversity threshold set at upper quartile of population distribution.

A custom atmospheric correction procedure for VSWIR hyperspectral data that uses ACORN-6LX software with 200-meter processing kernels incorporating local topography and view-angle geometry to account ...

Random collection of mayfly larvae from stream benthos using standardized D-frame nets, followed by preservation and microscopic examination for internal parasites. Used to compare parasite prevalence...

Yeast isolates were cultured on chloramphenicol-supplemented YM agar and identified through PCR amplification and sequencing of the D1/D2 domains of the large subunit nuclear ribosomal RNA gene using ...

Histochemical staining using DAB for hydrogen peroxide and NBT for superoxide detection in plant tissues following herbivore attack. Reveals spatial patterns of oxidative stress responses around feedi...

Analysis of citizen behavioral responses to government security threat level changes using polling data and government resource usage statistics. Used correlation and linear modeling to assess habitua...

Standard DNA extraction, amplification and sequencing of mitochondrial COI barcode region and nuclear PEPCK gene from museum and field specimens to generate phylogenetic data for species delimitation.

Combined morphological identification using dissecting microscopy with DNA barcoding for genetic confirmation of species identities and morphospecies designation.

Development of power-law scaling relationships between environmental variables and DOM chemodiversity using ordinary least squares regression on log-transformed data.

Daily forced flight protocol involving 3 × 4 minute flight bouts with rest periods, stimulated by gentle brushing, to experimentally increase energy expenditure in butterflies. Control group remains i...

Daily walking surveys along a road transect to detect, identify, tag and remove small mammal roadkill carcasses within hours of detection. Compared detection efficiency against simulated weekly survey...

Standardized ranking of vegetation cover in quadrats using the established Daubenmire cover class system.

Deep machine learning algorithm trained with ~29,000 images to automatically detect and flag time-lapse images potentially containing hummingbirds for manual verification.

Field surveys to validate species distribution model predictions by systematically searching grid cells for species presence/absence and comparing to model predictions using statistical performance me...

Non-invasive sampling method using swabs to collect dermal secretions containing corticosterone from amphibian skin for stress hormone analysis. This method was validated for use with axolotls and app...

Manual removal and dissection of flowers to detect and collect insects present inside flower structures, with specimen preservation for identification.

Application of d-excess calculations (δ²H - 8·δ¹⁸O) as a novel tracer for identifying high-elevation snowmelt contributions to streamflow based on elevation-dependent isotopic fractionation patterns.

Controlled exposure of D. geminata cells to different water sources with varying nutrient levels to test cellular division and mat formation responses. Involves transplanting cells between environment...

Use of digital photography combined with computer software to accurately count eggs and first instar larvae in butterfly egg clusters. Includes estimation methods for eggs in multiple layers.

Visual size estimation of treehopper nymphs using reference cards with actual nymphs of known sizes glued to cards, calibrated weekly with caliper measurements of sample nymphs.

Collection of dissolved noble gases from groundwater wells using copper tubes with down-hole pumps and back-pressure valves to prevent degassing during sample collection.

Systematic placement of monitoring devices along transects perpendicular to trails at fixed distances to assess the spatial effect of human disturbance on wildlife communities.

Deployment of low-cost temperature loggers across elevational transects to measure near-surface air temperature variation in complex terrain. Loggers positioned on north sides of trees for natural rad...

Collection of continuous vertical and horizontal drill cores from shale bedrock to characterize sulfur speciation changes with depth and weathering extent. Combines drilling techniques with subsequent...

Uses drone-mounted multispectral and thermal sensors to collect fine-scale habitat data within individual home ranges. Combines thermal imagery, vegetation mapping, and digital elevation models to qua...

Chemical clearing of Drosophila male genitalia using NaOH treatment, followed by microscopic imaging and principal component analysis of genital shape variation.

Microscopic examination of female Drosophila ovaries to determine reproductive state by assessing ovary size, transparency, and egg development stage to classify females as diapausing or reproductivel...

Plants are subjected to controlled drought conditions by restricting watering frequency and monitoring soil moisture levels. Treatment groups include normal watering, drought-stressed, and drought-rec...

Method to evaluate efficiency of microphysical schemes for converting water vapor flux to precipitation based on integrated vapor transport normalization. Uses atmospheric pressure and wind components...