DNA barcoding (Apidae)

Subcategory: DNA sequencing

Papers: 1 | Mentions: 1

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Description

Standard DNA extraction, amplification and sequencing of mitochondrial COI barcode region and nuclear PEPCK gene from museum and field specimens to generate phylogenetic data for species delimitation.

Typical Equipment

standard DNA extraction protocols
PCR primers
sequencing equipment
MEGA version 6.06
EMBOSS Transeq

Output Measurements

COI sequences
PEPCK sequences
unique haplotypes

Papers Using This Protocol (1)

article

Genes Suggest Ancestral Colour Polymorphisms Are Shared across Morphologically Cryptic Species in Arctic Bumblebees

2015PLoS Oneusing