Controlled freezing stress treatment (Liliaceae)

Subcategory: environmental stress

Papers: 1 | Mentions: 1

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Description

In situ freezing treatment using liquid nitrogen and heat exchanger to gradually cool plant tissues to -30°C. Designed to simulate natural freezing events while allowing controlled temperature ramping and monitoring.

Typical Equipment

cylindrical styrofoam chambers
70 L Dewar flask
coiled copper tubing heat exchanger
digital microprocessor thermometer

Output Measurements

leaf temperature
air temperature
freezing stress response

Papers Using This Protocol (1)

article

<i>In situ</i> photosynthetic freezing tolerance for plants exposed to a global warming manipulation in the Rocky Mountains, Colorado, USA

2004New Phytologistintroducing