D1/D2 rRNA gene sequencing (Ranunculaceae)

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Description

Yeast isolates were cultured on chloramphenicol-supplemented YM agar and identified through PCR amplification and sequencing of the D1/D2 domains of the large subunit nuclear ribosomal RNA gene using primers NL1 and NL4.

Typical Equipment

• YM agar
• chloramphenicol
• REDExtract-N-Amp Tissue PCR kit
• DNAEngine Thermal Cycler

Output Measurements

• yeast species identity
• DNA sequences

Papers Using This Protocol (1)