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Comparative anesthesia efficacy testing in amphibians
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Description
Standardized protocol for comparing anesthetic effectiveness by measuring induction and recovery times in salamanders using righting reflex as the endpoint. Animals are immersed in anesthetic solution until loss of righting reflex, then monitored through recovery phases.
Typical Equipment
- Sigma-Aldrich MS-222
- Sigma-Aldrich benzocaine
- rectangular plastic containers
- aged water
- stopwatch
Output Measurements
- induction time
- initial recovery time
- final recovery time
- mass change