DAB staining (Drosophilidae)

Subcategory: plant defense response

Standard reference: Clarke 2009

Papers: 1 | Mentions: 1

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Description

Histochemical staining using DAB for hydrogen peroxide and NBT for superoxide detection in plant tissues following herbivore attack. Reveals spatial patterns of oxidative stress responses around feeding damage sites.

Typical Equipment

3,3'-diaminobenzidine tetrahydrochloride (DAB)
nitro blue tetrazolium (NBT)
microscope

Output Measurements

H₂O₂ localization
superoxide localization

Papers Using This Protocol (1)

article

Mining the plant-insect interface with a leafmining <i>Drosophila</i> of <i>Arabidopsis</i>

2011Molecular Ecologyusing