Protocols

Rock fragments measured for magnetic hysteresis properties and isothermal remanent magnetization to identify magnetic mineral carriers and domain states.

Controlled trials placing two males of different sizes with one female in observation arenas to determine effects of male body size on reproductive success.

Analysis of recorded alarm vocalizations for nonlinear acoustic phenomena including subharmonics, biphonation, deterministic chaos, entropy and goodness of pitch measures.

Collection of fecal samples from wild yellow-bellied marmots during summer active seasons for bacterial community analysis.

A matched pair design comparing scientific productivity outcomes between REU participants and demographically similar non-participating applicants tracked over 5-7 years. Uses multiple data sources to...

Paired study design comparing contaminated mine sites with nearby uncontaminated control sites matched for similar vegetation patterns and environmental conditions. Multiple response variables measure...

Maximum likelihood approach to estimate relative resistances of different matrix types to butterfly movement, modeling movement as inverse power function of distance.

Used Maximum Entropy machine learning algorithm to predict species occurrence based on known presence locations and environmental variables, followed by independent field validation of model predictio...

Model-based clustering of morphological data using maximum likelihood methods with Bayesian Information Criterion for model selection, testing 1-9 cluster models to determine optimal population struct...

Computational approach for constructing phylogenetic trees of viral protein sequences using maximum likelihood methods with various substitution models and statistical support assessment.

Construction of phylogenetic trees from DNA sequence alignments using maximum likelihood method with HKY model and bootstrap analysis in PAUP*.

Caterpillars were enclosed in mesh bags on plant inflorescences to measure constitutive plant defenses while excluding natural enemies. Development time, pupal mass, mortality and parasitism rates wer...

Systematic literature review and quantitative synthesis examining how fungal symbionts modify plant responses to global change factors using effect size calculations and weighted linear models.

Systematic search, selection, and quantitative synthesis of studies examining age and sex differences in mammalian alarm call responses. Converts diverse study statistics to standardized effect sizes ...

Mathematical modeling approach extending classic Levins metapopulation models to incorporate both consumptive and nonconsumptive effects of generalist predators on prey colonization and extinction dyn...

Processing of PRISM daily precipitation and temperature data to derive snow-relevant variables through backward time accumulation from observation dates, including snowfall estimation and degree-day c...

Generalized Michaelis-Menten growth function modeling annual canopy volume change as a function of initial size and environmental covariates, with skewed-t measurement error to account for LiDAR proce...

DNA and RNA co-extraction followed by cDNA synthesis and next-generation sequencing to assess relative abundance of active versus dormant microbial operational taxonomic units. Uses ratio of rRNA to r...

Fine-scale temperature measurement using thermocouples positioned at stigma level within flowers to test whether different style morphs experience different thermal environments. Paired design compari...

Continuous monitoring of temperature at surface and burial depth using data loggers, combined with regular soil moisture measurements to characterize microhabitat conditions.

Visual counting and sizing of microplastic particles followed by FTIR spectroscopic confirmation of polymer composition.

Development of microsatellite primers through genomic library construction, enrichment, cloning, sequencing, and primer design for population genetic studies.

Systematic search of conifer forest patches to locate and classify red squirrel middens as active or abandoned, using midden presence as a proxy for squirrel population density.

Comparative field investigation of military training and battle-zone landscapes to understand relationships between terrain characteristics and combat effectiveness.

Captures dippers using mist nets across streams and applies unique color band combinations plus USGS metal bands for individual identification.

Mixed effects analysis of covariance testing effects of water availability and environmental covariates on nectar production and flower color. Includes multiple interaction terms and site as a factor.

Combined structured program documentation, focus groups, and surveys to evaluate remote undergraduate research program implementation and student experiences during COVID-19.

Monte Carlo approach to quantify uncertainty in terrestrial carbon cycle responses and determine plausible ranges of human emissions compatible with specific atmospheric CO2 concentrations. Incorporat...

Standardized measurements of wing chord, tail, tarsus, culmen, crown width, and crown stripe width were taken on captured birds using rulers and calipers to assess sexual dimorphism.

Mosquitoes paralyzed with triethylamine and identified morphologically under dissection microscope, with subset preserved at -80°C for molecular analysis including West Nile Virus RNA detection.

Integration of body mass data from multiple databases (Hayssen 2008, PANTHERIA) with habitat classification data from IUCN Red List to create a comprehensive dataset linking morphology and ecology.

Comprehensive site characterization including canopy light availability via hemispherical photography, disturbance assessment via cow presence indicators, regolith classification, and physiographic va...

Comprehensive arthropod sampling using wide range of collection methods from soil to forest canopy to capture taxonomic breadth of Arthropoda.

Integrated approach combining borehole drilling with comprehensive geophysical logging including NMR, electrical resistivity, temperature, and electromagnetic measurements to characterize subsurface b...

Systematic feature selection using six complementary statistical methods to identify key environmental drivers of chemodiversity while reducing bias from any single technique.

Comparative simulation study using five different soil organic carbon models (DAYCENT, CORPSE, MIMICS, MEND, RESOM) to project responses to warming and litter addition manipulations. Models were run u...

Protocol for evaluating antenna pointing accuracy and measurement comparability between multiple Doppler radar systems using solar alignment, constant elevation scans, and range height indicator measu...

A systematic approach to test climate change impacts at multiple model resolutions by running the same scenarios at both coarse (1 km) and fine (100 m) spatial resolution. Climate scenarios are develo...

Systematic comparison of watershed model performance using meteorological forcing datasets at different spatial and temporal resolutions. Evaluates how forcing resolution affects simulated hydrologic ...

A method for constructing plant-pollinator interaction networks at multiple temporal scales from temporally-explicit field observations. Networks are aggregated from daily observations to weekly, mont...

Standardized processing and quality control of aerosol measurements from multiple DOE/ARM sites to create consistent, intercomparable datasets. Includes size distribution harmonization, temporal avera...

Synthesis of climate records from five different sources (resident observer, NOAA, USGS, USDA, and PRISM) with standardized protocols for handling missing data and format inconsistencies. Includes cal...

Principal component analysis applied across multiple species using demographic traits to identify major axes of life-history variation and test for slow-fast continuum patterns. Uses phylogenetically-...

Integration of historical aerial photography, LiDAR data, and multispectral imagery to track channel migration and identify abandoned floodplain features using automated classification in eCognition s...

Decomposition of total seed production into four multiplicative components: flower number, fruit set, seed set, and seed escape from predation. Allows identification of which reproductive stages are m...

Large-scale examination of museum specimens to characterize geographic variation in polymorphic traits across species ranges

Novel ensemble learning approach combining XGBoost, CatBoost, and Random Forest algorithms with SHAP interpretability analysis to predict cloud condensation nuclei from aerosol optical properties.

High-resolution airborne hyperspectral and lidar data collection and analysis to classify land cover types and derive vegetation parameters for hydrologic modeling.

Experimental protocol to test for circadian rhythmicity in photoperiodic responses by exposing insects to constant day length (12h) with varying night lengths to create non-24h photoperiods, then meas...

Standardized collection of wet and dry atmospheric deposition samples using NADP Aerochem Metrics collectors to quantify microplastic fallout in remote areas.