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Microbial dormancy molecular analysis

Subcategory: molecular
Standard reference: Mueller and Hesse (2016)
Papers: 1 | Mentions: 1

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Description

DNA and RNA co-extraction followed by cDNA synthesis and next-generation sequencing to assess relative abundance of active versus dormant microbial operational taxonomic units. Uses ratio of rRNA to rDNA recovery to determine dormancy levels.

Typical Equipment

  • MoBio DNeasy kits
  • RNeasy kits
  • random hexamers
  • SMARTscribe Reverse Transcriptase Kit
  • next-generation sequencing platform

Output Measurements

  • relative abundance of active OTUs
  • relative abundance of dormant OTUs
  • rRNA:rDNA ratios

Papers Using This Protocol (1)

student_paper

Dormancy rates in microbial communities across an elevational gradient

2017using