Microsatellite enrichment and primer development (Polemoniaceae)

Subcategory: molecular

Standard reference: Glenn and Schable (2005)

Papers: 1 | Mentions: 1

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Description

Development of microsatellite primers through genomic library construction, enrichment, cloning, sequencing, and primer design for population genetic studies.

Typical Equipment

RsaI endonuclease
biotinylated oligonucleotides
TOPO TA Cloning vector
Escherichia coli TOP10 competent cells
Primer3 software

Output Measurements

microsatellite primer sequences
allele sizes
polymorphism metrics

Papers Using This Protocol (1)

article

Microsatellite loci in <i>Ipomopsis aggregata</i> (Polemoniaceae) and cross-species applicability for ecological genetics studies

2012American Journal of Botanyintroducing