Protocols

Standardized preparation of rock samples for weathering experiments including crushing to uniform size, ultrasonication cleaning to remove fines, and oven drying. Designed to expose fresh surface area...

PCR-based genotyping to identify sex chromosome haplotypes in the sex-determining region (SDR) of Fragaria virginiana, distinguishing between alpha, beta, and gamma variants.

Assessment of belowground seed bank viability and abundance through controlled greenhouse germination trials. Seeds collected from natural populations and tested under standardized conditions followin...

Laboratory leaching of soil and rock samples using artificial groundwater solutions to determine nitrogen and ion release potential. Samples are powdered and equilibrated with solution for chemical an...

Modified phenol-chloroform extraction protocol optimized for soil samples using CTAB buffer and mechanical disruption for simultaneous DNA and RNA recovery.

Two-stage sieving protocol using 2000-micron and 500-micron mesh sieves to separate seeds and seed fragments from soil samples, followed by visual identification using herbarium resources.

Standard plant histological protocol involving glutaraldehyde fixation, resin embedding, serial sectioning at 1.5 μm, and multiple staining techniques including toluidine blue for general structure an...

Surface sterilization and cultivation of leaf endophytes from grass blade segments using ethanol and sodium hypochlorite sterilization followed by plating on culture medium. Isolates were maintained u...

Cold stratification protocol for hemiparasite seeds followed by controlled germination under standardized temperature and photoperiod conditions. Includes seedling selection at specific developmental ...

Serial dilution of sonicated material samples plated on agar with selective media to enumerate and characterize fungal morphotypes while inhibiting bacterial growth.

Microscopic dissection to locate and examine spermatheca in female burying beetles. Standard entomological dissection technique adapted for reproductive organ examination.

Dissection and counting of spermatophores in female bursa copulatrix to quantify mating success and reproductive history in wild-caught butterflies.

Standard biochemical assay using 2,3,5-triphenyltetrazolium chloride staining to distinguish between dormant and nonviable seeds that failed to germinate.

Processing of sediment samples in anaerobic chamber with nitrogen-hydrogen gas mixture to preserve field conditions.

Cleaning, drying at 65°C for 72 hours, grinding, and powdering of foliar samples for analysis.

Laboratory analysis of blood samples including RIN score determination and cell counts.

Laboratory method for sample preparation and analysis.

Sample preparation with 2% nitric acid dilution and rigorous quality assurance and quality control processes including blanks, standards, and calibration.

Laboratory analysis of collected aeolian sediment samples to determine dust mass and horizontal flux.

Soil samples extracted into exchangeable, apatite, and silicate pools using different chemical solutions to isolate nutrient sources.

PCR-based method used to detect coronavirus sequences in wildlife samples collected from various points along supply chains.

Measurement of neutrophilic phagocytosis of fungal conidia using recombinant components.