Soil nucleic acid extraction with phenol-chloroform (Asteraceae)

Subcategory: molecular extraction

Papers: 1 | Mentions: 1

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Description

Modified phenol-chloroform extraction protocol optimized for soil samples using CTAB buffer and mechanical disruption for simultaneous DNA and RNA recovery.

Typical Equipment

2 ml Lysing Matrix E tube
MP Biomedicals FastPrep-24 instrument
AllPrep DNA/RNA Mini Kit

Output Measurements

purified DNA
purified RNA
nucleic acid concentrations

Papers Using This Protocol (1)

article

Virus diversity and activity is driven by snowmelt and host dynamics in a high-altitude watershed soil ecosystem

2023Microbiomeusing