Standard plant histology (Polemoniaceae)

Local Knowledge Graph (13 entities)

Description

Standard plant histological protocol involving glutaraldehyde fixation, resin embedding, serial sectioning at 1.5 μm, and multiple staining techniques including toluidine blue for general structure and specialized stains for cell wall components.

Typical Equipment

• 3% glutaraldehyde
• 0.1 M sodium cacodylate buffer
• ethanol series
• Spurr's resin
• microtome
• toluidine blue
• ether aniline blue
• phloroglucinol
• ruthenium red

Output Measurements

• cellular structure
• cell wall composition
• tissue organization

Papers Using This Protocol (1)