Surface sterilization and isolation of leaf endophytes (Poaceae)

Subcategory: microbiology

Papers: 1 | Mentions: 1

Local Knowledge Graph (9 entities)

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Description

Surface sterilization and cultivation of leaf endophytes from grass blade segments using ethanol and sodium hypochlorite sterilization followed by plating on culture medium. Isolates were maintained under controlled temperature conditions.

Typical Equipment

95% ethanol
1% sodium hypochlorite
70% ethanol
culture plates
plating medium

Output Measurements

culturable fungal isolates
morphological OTUs

Papers Using This Protocol (1)

student_paper

Understanding how leaf endophytes are affected by climate change: Examining fungi in grass species with warming

2016using