Protocols

A method for constructing plant-pollinator interaction networks at multiple temporal scales from temporally-explicit field observations. Networks are aggregated from daily observations to weekly, mont...

Standardized processing and quality control of aerosol measurements from multiple DOE/ARM sites to create consistent, intercomparable datasets. Includes size distribution harmonization, temporal avera...

Principal component analysis applied across multiple species using demographic traits to identify major axes of life-history variation and test for slow-fast continuum patterns. Uses phylogenetically-...

Integration of historical aerial photography, LiDAR data, and multispectral imagery to track channel migration and identify abandoned floodplain features using automated classification in eCognition s...

A computational model simulated bee foraging decisions based on nectar volume thresholds, moving theoretical bees through inflorescences to calculate net energy gain rates.

Compares non-nested linear models using only species identity as predictor versus nested mixed models that account for genotype variation within species. Evaluates model performance using R², AIC, and...

Statistical method comparing observed innovation diffusion patterns against social network structure to detect social transmission versus purely asocial learning.

Construction of trophic species webs from field observations followed by calculation of Link Area Relationships and comparison with the Constant Connectance in Space model predictions.

Digital signal processing technique using normalized spectrogram correlations to quantify acoustic signal degradation by comparing two-dimensional spectrograms between reference and experimental dista...

Iterative inversion technique for retrieving atmospheric parameters from contaminated radar measurements using a priori constraints and forward modeling. Accounts for measurement uncertainties and cro...

Preparation of polished sections of ore samples and detailed microscopic examination to determine mineral relationships, textures, and paragenetic sequences. Includes camera lucida drawing documentati...

DNA extraction from aphids followed by PCR amplification using specific primers for H. defensa and B. aphidicola, with gel electrophoresis visualization.

PERMANOVA and PCoA analyses based on Bray-Curtis dissimilarity to test for differences in ant community composition and visualize community structure changes through time and across treatments.

Standard PLFA extraction and analysis to characterize soil microbial community structure and biomass, with different fatty acids representing bacteria, fungi, and actinomycetes.

Standardized literature search and screening process to identify and synthesize studies on hibernating mammal responses to climate change, followed by categorization and statistical analysis of climat...

Inversion of the PROSPECT-5B radiative transfer model to estimate leaf transmittance from reflectance measurements for calculation of leaf absorptance across the 400-2,500 nm spectral range.

Integration of literature-derived parameter values with field data validation using long-term cohort observations from Mexican Cut, Colorado. Parameters include growth rates, consumption rates, matura...

Calculation of conservation concern scores using Partners in Flight rankings multiplied by species abundance for site-level conservation value assessment.

Statistical method to correct for inherent male bias when calculating sibling sex ratios and family sizes from male-only cohorts. Removes bias introduced by the inclusion of cohort members in family c...

Used phylogenetic generalized least squares (PGLS) regression and phylogenetic ANOVA to test relationships between acoustic call frequencies, body size, and habitat while controlling for evolutionary ...

Comprehensive analysis of the evolutionary history and phylogenetic distribution of herbivory, honeydew production, and ant-tending across hemipteran lineages. Constructed timeline of key evolutionary...

Nonlinear function fitting using seed=(k*pollen)/(β+pollen) where k represents maximum seed set and β represents saturation parameter, analyzed using SAS NLIN procedure.

A method for quantifying floral trait combinations and comparing them to idealized pollination syndromes using multidimensional scaling in phenotype space. Plant species are scored for 41 binary trait...

Dynamic snow identification algorithm using polynomial curves to classify snow pixels based on RGB color values. Adapts thresholds based on lighting conditions using red/blue ratios with additional wh...

Population genetic analysis using pooled sequencing data to estimate nucleotide diversity across chromosomes and validate sex chromosome identity through coverage analysis.

A framework for testing geological hypotheses using geophysical data that integrates Monte Carlo simulation, dimension reduction, and Bayesian updating to account for uncertainty in both model paramet...

Regression analysis of population density over time to quantify decline rates, followed by statistical comparison between treatment groups.

Estimation of natural loss rates using analysis of covariance on slopes of linear regressions from time series of ln-transformed larval densities.

Standardization of environmental variables measured on different scales followed by principal components analysis to reduce dimensionality and identify major axes of environmental variation.

A PCA was used to reassign values for growth, reproduction, and defense measurements, and ratios between these categories were calculated for each plant population.

Collection of outermost growth rings from dead wood samples, chemical pretreatment using AAA method, and accelerator mass spectrometry analysis to determine time since tree death. Calendar ages estima...

Computer-based randomization procedure to generate F-statistics and probability values for factorial designs when data cannot be normalized through transformation.

Species diversity technique adapted for song analysis that standardizes sampling effort across individuals to provide comparable repertoire size estimates using rarefaction curves.

Integrative model combining photosynthesis measurements, respiration estimates, and total nonstructural carbohydrate analysis to calculate carbon allocation to reproduction.

Measurement of head capsule widths across larval instars to assess morphological differentiation between coexisting Rhyacophila species, combined with analysis of species coexistence patterns and prey...

Point pattern analysis using Ripley's K function to test for clustering, randomness, or regularity in tree spatial distributions by comparing observed patterns to simulated random distributions.

Mica sheets are placed over thin sections, irradiated in a nuclear reactor, and examined under microscopy to identify areas of elevated uranium concentration through fission track density patterns. Th...

A novel method that combines FT-IR and bulk carbon XAS spectroscopy results using a linear mass balance model constrained by elemental analysis to quantify the abundance of seven SOC functional groups...

Novel application of hierarchical k-means clustering to cumulative distribution curves of Standard Precipitation-Evapotranspiration Index to identify climate zones. Uses temporal segmentation to detec...

Rigorous matching protocol for satellite and ground-based observations accounting for viewing geometry effects. Applies parallax correction to compensate for apparent cloud displacement and uses stric...

Quantifies temporal overlap in flowering periods between species pairs using Schoener's index, where values approach 1 when flowering curves coincide and approach 0 when they don't overlap.

Calculation of Shannon-Wiener Diversity Index for each site to determine differences in species richness and relative abundance, using relative cover as the abundance metric.

Uses Advanced Land Observing Satellite-1 Interferometric Synthetic Aperture Radar data with Short Baseline Subset processing to detect and quantify very slow landslide movements over multi-year period...

Novel analytical framework incorporating individual size variation into niche overlap calculations to predict potential for competition versus intraguild predation between species pairs.

A computational approach to test the stability of social network parameters by systematically removing increasing percentages of observational data and recalculating network measures to determine sens...

Complete digestion of rock and soil samples using HF/HNO₃ followed by ion exchange chromatography separation for rhenium analysis by mass spectrometry.

Statistical analysis using Moran's I to detect spatial patterns in allele frequencies across distance classes. Calculates correlation coefficients for genetic similarity as a function of geographic di...

Systematic manipulation of invasive species spatial distribution patterns varying coverage, patch number, size, shape, and distances. Statistical analysis of resulting population responses using linea...

Validation of theoretical SAR predictions by fitting log-log regressions to existing state-level plant species count data across the continental United States.

Standardized Precipitation Evapotranspiration Index calculated over 12-month water-year periods to quantify drought severity, incorporating both precipitation and temperature effects on water balance.