PCR amplification of bacterial endosymbionts (Aphididae)

Local Knowledge Graph (9 entities)

Description

DNA extraction from aphids followed by PCR amplification using specific primers for H. defensa and B. aphidicola, with gel electrophoresis visualization.

Typical Equipment

• PCR thermal cycler
• agarose gel electrophoresis
• EtBr staining

Output Measurements

• presence/absence of H. defensa
• presence/absence of B. aphidicola

Papers Using This Protocol (1)