Protocols

Uses ordinal logistic regression to model the probability of experiencing different levels of outdoor recreation constraints based on sociodemographic characteristics. Allows for multiple response cat...

Statistical fitting of dose-response curves to photoperiodic data using log-logistic models to estimate the critical day length (CDL) where 50% of females enter diapause.

Fitting both linear and nonlinear piecewise regression models to phenological response data, using AIC for model selection and requiring minimum data points on either side of breakpoints.

Used mixed-effects SEM framework to determine direct and indirect effects of climate and density-dependent variables on larval body size and morph outcome. Models incorporated random effects for cohor...

Quantitative PCR using bacterial-specific primers to measure bacterial load in leaf tissue samples across plant patches

Estimates historical fire rotation as 2-3 times the post-fire recovery period for sagebrush to return to pre-fire conditions, based on recovery studies.

Body condition assessment using regression residuals of weight against log-transformed length, following Jakob et al. (1996) as the most reliable method for behavioral studies

Taxonomic classification of microbial communities using ribosomal protein S3 (rpS3) marker genes identified through Hidden Markov Models and phylogenetic analysis.

Uses historical government land survey records from 1876-1892 to reconstruct fire locations and calculate fire rotation based on burned area and observation period.

Standard skeletochronology protocol involving tissue fixation, decalcification, cryostat sectioning, histological staining, and LAG counting to determine age in amphibians.

Comparative analysis of soil development across surfaces of different ages to establish relative chronology. Involves detailed morphological description and mechanical composition analysis of soil hor...

Carbon and nitrogen stable isotope analysis of ant tissues to determine trophic position and dietary composition. Involves specimen preparation, drying, grinding, and mass spectrometry analysis.

Statistical method using the strucchange package in R to detect structural changes in meteorological time series by evaluating F-statistics and identifying optimal breakpoints using dynamic programmin...

Confirmatory path analysis using structural equation modeling to test hypothesized direct and indirect causal relationships between social factors, behavioral traits, and problem-solving success. Uses...

Calculated visibility from specific locations across a landscape using digital elevation models and GIS software to determine areas visible from observation points.

454 sequencing to characterize genetic diversity at the DRB Class II locus in montane voles.

Akaike Information Criterion weighted ensemble multiple linear regression framework using 47 watershed traits to predict snow-to-streamflow relationships.

Full raw argon isotopic data collection and analysis conducted in the Argon Geochronology Laboratory of the USGS in Denver, Colorado.

Correction of pressure transducer data by subtracting absolute atmospheric pressure measured by barometers or weather stations.

Spectroscopic technique for quantitative determination of chemical elements in samples.

Mathematical modeling approach to analyze prey selection patterns.

Uses xESMF package to interpolate different UAS measurements to a common rectilinear grid, with bilinear interpolation for soil moisture and conservative interpolation for other variables.

Statistical model used to analyze current reproduction probability in relation to previous reproductive history.

Image processing system used to classify and analyze camera trap photographs for detection data extraction.

Cellulose extraction using Brendel method followed by pyrolysis at 1400°C and isotopic ratio measurement by GC-IRMS.

Climate forcing using minimum and maximum daily temperature lapse rates defined by Snow Telemetry stations adjusted for aspect.

Temperature lapse rate adjustments using SNOTEL stations and PRISM rainfall distribution for model input.

Analysis of tungsten by colorimetric methods.

Data cleaning procedures including interpolation of missing values and setting snow depths to zero after depletion date.

Statistical analysis to detect intraspecific negative density dependence during seed-to-seedling transition.

Three dominance scores calculated for each species at each site where it occurred to assess competitive hierarchies.

Processing of ERT measurements based on data quality observations across the monitoring period.

Quality control analysis comparing energy balance closure with friction velocity values to ensure data quality.

Data preparation phase involving exploratory analysis of meteorological parameters from multiple databases.

Fourier transform infrared spectroscopy analysis of organic residues from archaeological samples.

Chemical analysis method for determining precious metal concentrations in mineral samples.

Examination of fish digestive systems to identify and quantify ingested microplastic particles. Analysis method for studying microplastic ingestion by marine life.

Correlation of daily flows with USGS stream gauge data and linear interpolation for ice-covered periods.

Laboratory technique to determine ploidy level, validated against Hordeum vulgare standards.

Flow cytometer measurement of live cell counts for bioaerosol particles collected during 24-hour sampling period.

Ammonia determination using Lachat QuikChem flow injection system with colorimetric detection at 660 nm.

Analytical technique using fluorescence properties to measure chemical concentrations.

Analysis of natural erosion effects on archaeological deposits including size-sorting of artifacts and postdepositional disturbance by water and wind.

Assessment of the amount and character of native Fremont cottonwood forest remaining on the mainstem floodplains in 26 subbasins using geographical information system analysis.

Using ArcGIS to digitize habitat polygons and generate random sampling points within appropriate community types.

Methane concentrations measured using headspace equilibration technique and gas chromatograph with flame ionization detector.

Spatial interpolation of mineral and organic matter concentrations from monitoring well data to represent geochemical heterogeneity.

GLMs and GLMMs used to model periodic differences in vital rates for each life-cycle stage, testing for effects of season or post-fire habitat states.

GS approach using SNP markers to predict genetic values and build selection models.

Chemical analysis of igneous and meta-igneous rock samples to determine composition and petrogenesis. Results presented in tables and geochemical diagrams.