skeletochronology (Ambystomatidae)

Subcategory: histological age analysis

Standard reference: Castanet and Smirina, 1990

Papers: 1 | Mentions: 1

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Description

Standard skeletochronology protocol involving tissue fixation, decalcification, cryostat sectioning, histological staining, and LAG counting to determine age in amphibians.

Typical Equipment

70% ethanol
3% nitric acid
cryostat (HM 505 E)
Harris' haematoxylin stain
phase-contrast microscope

Output Measurements

estimated age in years
LAG count

Papers Using This Protocol (1)

article

Accuracy assessment of skeletochronology in the Arizona tiger salamander (<i>Ambystoma tigrinum nebulosum</i>)

2007Copeiausing