Protocols

Conductivity measured with a Thermo Sci Orion DuraProbe 4-Electrode conductivity cell immediately after water extraction.

Pooled regression techniques applied to different time periods (1987-2011 vs 2012-2024) to evaluate shifting predictor variable importance.

Replicated split-plot design with snowmelt manipulated at plot level and precipitation manipulated at subplot level.

Network analysis to identify conflict among genes and show specific relationships that are inconsistent with each other.

Measurement of hydrogen and oxygen isotope ratios using Off-Axis Integrated Cavity Output Spectrometer with auto-sampler and heated injector block. Results reported in δ-notation relative to Vienna St...

Samples filtered through 0.22 μm Sterivex filters with housings made of Eastar co-polyester.

Installation of paired wells at nine locations to capture emerging groundwater from left and right banks.

Images were processed in Agisoft Metashape Software to derive a georeferenced dense point cloud and 3D model, then orthorectified and combined into a mosaic.

Sub-basins constructed using 10m digital elevation model from USGS National Elevation Dataset and ArcGIS Hydrology toolbox. Areas contributing flow to stream gage locations were delineated.

Attributing sub-watershed polygons with species presence, origin, seasonal and reproductive use information.

Qualitative collection using surface tows in estuarine and marine environments with volunteer monitoring.

Collection of surface water samples from 17 sampling sites within Fraser River from September 2021 through August 2023.

Coordinated sampling across multiple sites to quantify stream metal loads.

Deposition of particles on 400 mesh copper carbon-B film TEM grids for microscopy analysis.

Normalization of transcriptomic data using Transcripts Per Million (TPM) method.

Taxonomic nomenclature was standardized using TNRS iPlant Collaborative Version 4.0 to ensure consistent taxon names throughout the dataset.

Temperature measured using a Digi-Sense Thermistor probe routed to the bottom of wells with 1-minute stabilization.

Downscaling of daily or 6-hourly meteorological data to hourly frequency for hydrological modeling applications.

Statistical processing of discharge and chemical concentration data using R libraries for missing data interpolation and anomaly detection.

Rain measurements recorded via tipping bucket rain gauge, though may not be accurate for winter precipitation events like snow.

Creation of styled basemap depicting topographic slope and aspect using rainbow color scale with contour lines optimized for web display.

Transcriptomic analysis of whirling disease-resistant rainbow trout to understand gene expression patterns related to resistance.

Analysis method that reveals demographic and trait mediated processes that buffer population growth using transient life table response experiments.

Annual monitoring of plants in PVC pots including spring flowering/fruiting observations and August exhumation for corm weighing, morphology characterization, and photography.

Compared tree communities of contiguous and fragmented tropical rainforests to assess fragmentation effects.

Collection and voucher preparation of holotype and paratype specimens for taxonomic description.

Soil moisture measurements using the Lobe Differencing Correlation Radiometer (LDCR) L-Band microwave radiometer flown on aerial platforms.

UAV flights using DJI Mavic 2 Pro collecting RGB images with 3cm ground sample distance. Images processed via Structure from Motion in Agisoft Metashape to create georeferenced orthomosaics.

UAV flights conducted at altitudes ranging from 10 m to 120 m above ground level, with images systematically captured at 10 m intervals using DJI Air 2S equipped with 1-inch CMOS image sensor.

Regional assessments of major aquifer systems providing quantitative assessments of water occurrence, movement, and availability. Includes classification of stratigraphic sequences and generation of m...

Collection of gage height values using U.S. Geological Survey streamflow-gaging station methods. Data collected from station 09152500 during specified periods.

Records the UTM Y Coordinate for every pixel using the WGS84 UTM Zone 13N Coordinate System (EPSG:32613).

Use of Sonosite portable ultrasound machine to determine uterine litter sizes in late pregnancy for prairie dogs.

Collection of sea surface temperature, surface current, wind, and atmospheric pressure data from racing yacht during circumnavigation. Data collected using GPS, temperature sensors, Doppler velocity l...

Used to de-stagger mass and wind fields, interpolate forecast data to flight levels AGL and compute diagnostics such as visibility, ceiling height, and radar reflectivity.

Compilation of water-level information including depth to water, potentiometric-surface altitude, and saturated thickness from measurements by multiple agencies. Values were computed from raster datas...

Collection and analysis of discrete water quality samples including pesticide analysis. Results include load calculations and water quality parameters.

R script analysis of sex differences in integrated water use efficiency and population operational sex ratios in Valeriana edulis.

Continuous measurement of isotopic ratio of water vapor using Picarro L2130-i isotopic analyzer at EPA trailer in Gothic.

Data used to drive model simulations are downloaded from websites using Watershed Workflow software package. Model outputs are post-processed using Jupyter notebook scripts.

Processing waveform LiDAR data using Optech LMS software and custom IDL waveform processor to synchronize GPS time tags and geolocate waveforms.

Compilation of regolith thickness and bedrock altitude data from lithologic logs of wells and boreholes. Data was compiled from multiple agencies including Colorado Division of Water Resources, U.S. G...

Generation of whole genome sequences for Greater and Gunnison Sage-grouse to examine patterns of adaptive genetic variation.

Uses WIBS-Neo (DMT) to detect fluorescent bioaerosol particles (0.5-30 microns) based on excitation at 280 nm and 370 nm with emission detection at 310-400 nm and 420-650 nm wavelengths. Classifies pa...

Continuous coring of boreholes using wireline system to sample Quaternary colluvium and Cretaceous Mesaverde Formation.

CSU X-band Precipitation Radar used to classify hydrometeor types using fuzzy logic and semisupervised methods during the SAIL campaign.

DNA sequencing and analysis of Z-linked loci to assess genetic divergence and effective population sizes.

High-density airborne LiDAR scans collected in August-September 2015 and 2019 to generate normalized point clouds.

Aeromagnetic data were collected along flight lines by instruments in an aircraft that recorded magnetic-field values and locations. This dataset presents latitude, longitude, altitude, and magnetic-f...

Continuous data collection using automated instruments at monitoring stations.