Protocols

454 sequencing to characterize genetic diversity at the DRB Class II locus in montane voles.

Continuous monitoring of atmospheric black carbon and brown carbon mass loadings using a Magee Scientific aethalometer.

Akaike Information Criterion weighted ensemble multiple linear regression framework using 47 watershed traits to predict snow-to-streamflow relationships.

Automatic Identification Systems tracking vessel location and characteristics in real-time for traffic density analysis.

Collection of VSWIR field spectra at leaf- and mineral-levels using an Analytical Spectral Devices FieldSpec 3 spectroradiometer with contact probe and leaf clip attachments.

Remote sensing of atmospheric infrared radiances using Atmospheric Sounder Spectrometer by Infrared Spectral Technology (ASSIST) in wavenumber range 612-905.4 cm-1.

Uses ASTER thermal infrared data to identify areas of anomalous surface temperature. Areas with modeled temperatures between 1° and 2° above the mean are classified as weakly anomalous to anomalous.

Identification and mapping of active fault traces and structural features related to ongoing tectonic processes.

Data acquired using a Riegl Q1560 dual-channel LiDAR system mounted on a Piper Navajo aircraft. Survey complied with USGS QL1 standard with point density more than 8 pulses per m2.

Processing of sediment samples in anaerobic chamber with nitrogen-hydrogen gas mixture to preserve field conditions.

Annual counting of American white pelican colonies to monitor population dynamics at Gunnison Island.

Climate modeling to support archaeological interpretation of past environmental conditions.

Comprehensive archaeological survey and research including reconnaissance, systematic survey, and site evaluation/testing. Long-range program designed to understand area prehistory and adaptive patter...

Full raw argon isotopic data collection and analysis conducted in the Argon Geochronology Laboratory of the USGS in Denver, Colorado.

Two independent ANN models trained to separate land cover types and distinguish between cytotypes using hyperspectral data.

Calculation of fraction of Asian-sourced lead using mixing model based on lead isotope ratios.

Correction of pressure transducer data by subtracting absolute atmospheric pressure measured by barometers or weather stations.

Continuous measurements of surface energy budget, momentum flux, meteorology and soil properties using an atmospheric surface flux station. Data collected at 1-minute averages and 20 Hz resolution for...

Spectroscopic technique for quantitative determination of chemical elements in samples.

Long-term monitoring using Big Springs Number Eight dust samplers to measure seasonally accumulated horizontal sediment flux. Sample collections ranging from 1998 to present across varying landscapes.

Water level elevations obtained using barometric pressure from pressure transducers in wells, combined with TOC elevation and depth measurements.

Basin characterization modeling to provide watershed attribute data for SPARROW model input.

Anaerobic incubation of hyporheic sediments with artificial river water for 57 days to study kinetics of Fe(II), Mn(II), DOC and nitrate. Conducted in sealed bottles under N2 atmosphere.

Mathematical modeling approach to analyze prey selection patterns.

Uses xESMF package to interpolate different UAS measurements to a common rectilinear grid, with bilinear interpolation for soil moisture and conservative interpolation for other variables.

Statistical model used to analyze current reproduction probability in relation to previous reproductive history.

Assessment of reproduction traits in native and introduced ranges across 7-10 populations from both ranges.

Drilling of boreholes in 2017 and 2018 and completion as vertically discrete nested monitoring wells and single monitoring wells. Includes well construction documentation.

Quarterly collection of branchfall to assess branch turnover productivity.

USGS bridge crane with sounding weights used for streambed observations below the water line during cross-section surveys.

Rearing monarch butterflies on roadside- and prairie-collected milkweed to test effects of road salt runoff on development.

Blood sample collection following Clinical and Laboratory Standards Institute guidelines using EDTA anticoagulant tubes.

Assessment of Candidatus Mycoplasma haemominutum prevalence in domestic and wild felid populations.

Probes were calibrated annually in the field with CO2 standards and returned to manufacturer for factory calibration three times during study period.

Image processing system used to classify and analyze camera trap photographs for detection data extraction.

Automated data collection and storage from pressure transducers using Campbell Instruments data loggers.

Cellulose extraction using Brendel method followed by pyrolysis at 1400°C and isotopic ratio measurement by GC-IRMS.

Field sampling of logging roads abandoned between 1985-2015 to assess vegetation recovery in road track, edge, and adjacent forest zones.

Climate forcing using minimum and maximum daily temperature lapse rates defined by Snow Telemetry stations adjusted for aspect.

Temperature lapse rate adjustments using SNOTEL stations and PRISM rainfall distribution for model input.

Python implementation of algorithm from Lundquist et al., 2008, applied to digital elevation models to classify cold-air pooling areas using terrain analysis.

Annual counting and demographic assessment of prairie dog colonies including counts of sexually mature and immature individuals by age class.

Analysis of tungsten by colorimetric methods.

Endmember spectral measurements using contact probe attachment with internal bulb illumination for close-contact surface measurements.

Continuous injection of sodium bromide at the head of study reach for chemical tracer studies.

CNN training using image patches for pixel-based tree and shrub species classification at alpine treeline.

Field experiments creating F2 and F3 crop-weed hybrid lineages of genetically engineered rice with transgene constructs to test fitness effects under natural conditions.

Quantification of current velocity reduction by tidal marsh vegetation across distances from marsh edge.

Gap-filling areas outside Upper East River domain with interpolated data from 3m ASO DEM, harmonized with NEON DEM and filtered to remove small pits and peaks.

Guidelines for developing citations for Earth and environmental science data generated by the Earth Science Information Partners (ESIP).