Protocols

Integration of new and compiled geologic mapping data with geospatial analysis for neotectonic and hydrologic investigations.

Comprehensive scientific assessment of the state of knowledge regarding biodiversity, ecosystems and their contributions to people.

Carbon dioxide, nitrous oxide, and methane greenhouse gas concentrations and stable isotope signatures of CO2 and dissolved inorganic carbon were measured.

Calculation of narrow-sense heritability estimates and genetic correlations for growth traits and stem straightness.

Assembly and analysis of historical stocking records to document human-mediated changes in fish distribution.

Collection of site spectra to validate airborne hyperspectral data acquired by NEON Aerial Observation Platform.

Low-coverage genome sequencing using Illumina paired-end technology to generate draft genome assemblies for bird species.

Deriving individual-scale species data from co-acquired imaging spectrometry data.

Total carbon and nitrogen analysis using LECO TruSpec CN analyzer for decomposed needle samples.

Instrument responsivity calibrations performed using laboratory lamp calibrations for the MFRSR and in-field side-by-side calibration for the MFR.

Statistical approach using generalized Wishart probability distribution to identify landscape features affecting gene flow and estimate connectivity.

Laser ablation inductively coupled plasma mass spectrometry used for U-Pb detrital zircon geochronology analysis. Supports mapping and stratigraphic interpretations of Paleozoic sedimentary rocks.

Analysis using Picarro L2130-i or Los Gatos Research LWIA with cavity ringdown detection for stable isotope analysis.

Analysis of lead isotopic compositions using Neptune Plus multi-collector ICP source mass spectrometer with Tl spiking for mass fractionation correction.

Estimation of invertebrate biomass using established taxon-specific length-mass relationships and scaling to aerial measures.

Statistical analysis to quantify relationships between fecal microbial composition, diversity and social network traits.

Statistical method used to fill missing data areas using relationships between 2018 and 2019 snow depths or snow depth to elevation relationships.

Comprehensive literature review to compile carbon content data from tree tissues across multiple studies and species.

Annotation method for identifying and characterizing mitochondrial genes and features.

Processing of MODIS satellite data to derive surface albedo climatology using Google Earth Engine.

Digitization of analog contour maps by following lines or using intersection points of contours and flight lines.

Manual contouring of regolith thickness values to create isopach contours showing generalized thickness of sediments overlying bedrock.

Analysis of noble gases and tritium using mass spectrometry including helium in-growth method.

Statistical models used to infer mating parameters and pollen dispersal characteristics.

Dissolved sulfide concentration determined by reacting H2S with N,N-dimethyl-p-phenylene diamine under acidic conditions to form methylene blue.

Statistical analysis using mixed models to test for differences in network indices across seasonal periods.

Model selection analyses to evaluate possible transmission pathways including indirect vector transmission and direct predation transmission.

Specimens were analyzed using 18S and COI gene sequencing for molecular characterization.

Method used to root phylogenetic trees when outgroup taxa create long-branch attraction problems.

Analysis of samples for mineral composition, element composition, and nitrogen chemistry at multiple laboratories.

Statistical modeling approach to estimate salinity and selenium loads using geospatial and water-quality data as predictors.

Sequencing of 648 or 889 base pair region of the ND2 gene for at least 10 individuals from 53 contemporary populations to determine haplotype variation.

Sequencing of mitochondrial ND2 gene regions for phylogenetic analysis of cutthroat trout lineages.

Calculation of Normalized Differential Vegetation Index from 4-band aerial imagery using (NIR-Red)/(NIR+Red) formula.

Sample day by species matrix preparation for Non-metric Multidimensional Scaling analysis of vouchered bee data.

Nuclear magnetic resonance data processed to determine T2 relaxation time distribution for water content and geometric mean calculations.

Compilation and processing of NOAA weather data for degree-day calculations from 1950-2015. Temperature data aggregation for thermal ecology analysis.

Used eCognition software with RGB bands and normalized difference water index (NDWI) to classify channel water surface from imagery.

USGS Central Region Energy Team method for assessing oil and gas resources by defining provinces, Total Petroleum Systems, and Assessment Units based on geological characteristics.

Geometric processing method required to convert raw-space data to map-space surface reflectance.

Overlap estimates used to evaluate temporal changes within and between elevations.

Statistical testing of contribution of season, depth and location on viral community structure.

Data cleaning and post-correction process to remove bad values from raw POPS measurements, providing ready-to-use datasets.

Statistical method used to determine the most plausible branching schemes linking taxonomic groups.

Genetic analysis using nuclear microsatellite markers to determine paternal parentage and pollen dispersal patterns.

Derivation of hydraulic permeability and porosity from ERT data through petrophysical relationships for model parameterization.

Analysis of evolutionary relationships and taxonomic classification to understand angiosperm radiation into freezing environments.

Analysis of mitochondrial DNA, nuclear DNA, and morphological data using parsimony, maximum likelihood, and Bayesian methods.

Used to examine evolution of leaf economics spectrum across 28 species of Helianthus across environmental gradients.

Used to investigate evolutionary pathways of traits and climatic niches and their effect on diversification rates.