Protocols

Analysis of concatenated genetic data using phylogenetic mixture models to investigate evolutionary relationships.

Identification methods for marine phytoplankton including counts and abundance ratios of over 50 genera.

Analysis of pollen from archaeological site sediments to reconstruct past vegetation.

Analysis of plastic samples to determine polymer composition and material properties.

Generation of contours showing the generalized altitude of the potentiometric surface for the shallow groundwater system. Contours were created from values in the raster dataset potalt.

Mapping and zonation based on Principal Component Analysis and hierarchical clustering of site locations.

Systematic evaluation of data quality and calibration survey results for acquired remote sensing data.

Quantification of radon-222 concentrations using RAD7 instrument with closed loop sparging system and counting periods.

Python-based analysis combining field sampling and remote sensing data to estimate spatial distribution of soil thickness.

Statistical analysis using subset of main data to assess repeatability of measurements.

Spatially Continuous Riverbank Erosion and Accretion Measurements (SCREAM) software used to analyze channel masks and create centerlines and change area rasters.

Scanning electron microscopy with cathodoluminescence imaging for characterizing zircon internal structure.

Genotyping using a panel of 384 candidate-gene single nucleotide polymorphisms putatively associated with adaptive traits.

Surface Quantitative Precipitation Estimate product development using ARM-supported radar for snowfall quantification.

Composite gridded-image derived from 8-km resolution SST observations collected by AVHRR and HIRS instruments onboard NOAA and Metop-A satellites, generated every 48 hours for North America at 14 km r...

Examination of collected substrates to evaluate particle loading, morphology, and spatial distribution.

Marine scientists assign flags to data sets and WOCE flags of 2 (good), 3 (questionable) or 4 (bad) to individual fCO2 values during secondary quality control.

Development of sediment rating curves to characterize sediment transport in the reservoir system.

Data ranges for each sensor type were filtered based on a combination of sensor specifications and assumed reasonable values.

R code implementation for normalizing methylation data from the Mammalian Methylation Consortium.

Remote sensing analysis using Landsat Red and Green bands calculated as (Red - Green) / (Red + Green) to characterize soil properties.

Visualization of soil texture data using soiltexture package in R.

Sample pre-treatment method using PPL cartridges (Bond Elut) after acidifying samples to pH 2 with phosphoric acid to extract organic compounds.

Computational method for extracting velocity measurements from time-series image data.

Pooled regression techniques applied to different time periods (1987-2011 vs 2012-2024) to evaluate shifting predictor variable importance.

Network analysis to identify conflict among genes and show specific relationships that are inconsistent with each other.

Measurement of hydrogen and oxygen isotope ratios using Off-Axis Integrated Cavity Output Spectrometer with auto-sampler and heated injector block. Results reported in δ-notation relative to Vienna St...

Images were processed in Agisoft Metashape Software to derive a georeferenced dense point cloud and 3D model, then orthorectified and combined into a mosaic.

Sub-basins constructed using 10m digital elevation model from USGS National Elevation Dataset and ArcGIS Hydrology toolbox. Areas contributing flow to stream gage locations were delineated.

Attributing sub-watershed polygons with species presence, origin, seasonal and reproductive use information.

Taxonomic nomenclature was standardized using TNRS iPlant Collaborative Version 4.0 to ensure consistent taxon names throughout the dataset.

Transcriptomic analysis of whirling disease-resistant rainbow trout to understand gene expression patterns related to resistance.

Analysis method that reveals demographic and trait mediated processes that buffer population growth using transient life table response experiments.

Regional assessments of major aquifer systems providing quantitative assessments of water occurrence, movement, and availability. Includes classification of stratigraphic sequences and generation of m...

R script analysis of sex differences in integrated water use efficiency and population operational sex ratios in Valeriana edulis.

Processing waveform LiDAR data using Optech LMS software and custom IDL waveform processor to synchronize GPS time tags and geolocate waveforms.

Generation of whole genome sequences for Greater and Gunnison Sage-grouse to examine patterns of adaptive genetic variation.

DNA sequencing and analysis of Z-linked loci to assess genetic divergence and effective population sizes.

Analysis of autosome SNP genotypes using Arlequin input format for three grouse populations.

Resampling of 0.6m resolution imagery to 1m grid resolution using bilinear interpolation.

Creation of a binary map to define spatial boundaries of the study domain.

Calculation of carbon sequestration and biomass production in new trees and short-rotation coppice over 25 years.

Assessment of host range determinants using phylogenetic and functional trait approaches across 18 Asclepias species.

Method exploring the impact of individual and group social phenotypes on individual fitness relative to each other.

Used entropy techniques to identify temporal variability in geochemical constituents and their controls.

Systematic ranking of high-priority hazardous faults based on risk, characterization needs, and research potential.

Microscopic analysis of MASP binding to fungal pathogens using fluorescence techniques.

Gap-filling methods applied to daily and yearly in-stream carbon and nitrogen concentrations, discharge, and calculated exports.

One-dimensional hydraulic model was calibrated to determine the inundation necessary to inundate each plot and combined with flow data to determine inundation duration.

Analysis of data from Servicio Agrícola Ganadero of Chile describing geographic origin and importation records of bumblebee colonies and queens.