Protocols

Comprehensive measurement of vegetative traits including specific leaf area, trichome density, water use efficiency, and percent water content from field-collected leaves using scanning, drying, weigh...

Continuous collection of local weather data at 5-minute intervals throughout growing seasons to capture fine-scale environmental variation affecting disease dynamics.

Meta-analytical approach to synthesize population-level climate sensitivities across multiple species using structured population models and standardized effect sizes. Incorporates life history traits...

A novel approach to measure light response curves for entire plant communities rather than individual plants using tent-based gas exchange measurements across multiple light levels. This method contro...

Standard litterbag method deployed across elevation gradient to study litter decomposition rates and microbial succession. Involves placing known masses of litter in mesh bags and retrieving over time...

Systematic establishment of gridded plots across watersheds followed by long-term monitoring of tagged individual trees for mortality and recruitment rates. Combined with environmental characterizatio...

Cytotype inference using twelve multinational microsatellites with 97% correct classification for cytotype. Individual plants defined as diploid or triploid based on maximum number of alleles observed...

Comprehensive measurement of soil nutrients, moisture, pH, topographic aspect, and interpolated climate variables to characterize abiotic conditions at vegetation sampling sites.

PLSR modeling approach using leaf spectral reflectance measurements to extrapolate functional trait values from subset of direct measurements to larger sample sizes. Ten-fold cross-validation used for...

Experimental manipulation of natural floral orientations combined with pollen supplementation to test effects on seed set and female fitness.

Field experiment testing seed germination of different genotypes/treatments in natural microhabitats of related species to assess niche requirements and differentiation.

Maximum likelihood phylogenetic analysis of full genome sequences using MUSCLE alignment and Tamura-Nei evolutionary model to determine relationships between outbreak isolates and assess overwintering...

Seed-burial and greenhouse germination experiments to estimate seed bank-related vital rates including seed germination and stasis.

Direct measurement of aboveground biomass on 673 trees across five tropical countries. Compilation of tree mass dataset including 101 trees greater than 100 cm in diameter.