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Flow cytometry nuclear DNA content analysis (Salicaceae)

Subcategory: molecular
Standard reference: Blonder et al. 2020
Papers: 5 | Mentions: 6

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Description

Cytotype inference using twelve multinational microsatellites with 97% correct classification for cytotype. Individual plants defined as diploid or triploid based on maximum number of alleles observed across markers.

Typical Equipment

  • microsatellite markers
  • DNA extraction equipment
  • PCR amplification equipment

Output Measurements

  • cytotype classification
  • genotype identification

Papers Using This Protocol (6)

article

Relaxation of the leaf economics spectrum within and across quaking aspen Populus tremuloides genotypes

2025Oikosusing
article

Climate lags and genetics determine phenology in quaking aspen (Populus tremuloides)

2023New Phytologistusing
article

Remote sensing of cytotype and its consequences for canopy damage in quaking aspen

2022Global Change Biologyusing
article

Cytotype and genotype predict mortality and recruitment in Colorado quaking aspen (<i> Populus tremuloides </i>)

2021Ecological Applicationsintroducing
article

Cytotype and genotype predict mortality and recruitment in Colorado quaking aspen (<i> Populus tremuloides </i>)

2021Ecological Applicationsusing
article

Remote sensing of ploidy level in quaking aspen (<i>Populus tremuloides</i> Michx.)

2020Journal of Ecologyusing