Protocols
7 protocols
instar staging
Standardized measurements of wing length and dried body mass components under stereomicroscope to quantify adult fitness proxies and assess elevation effects on body size.
Multi-parameter water quality monitoring (Amphibia)
In-situ measurement of temperature, pH, dissolved oxygen, and barometric pressure at multiple points around each pond during peak photosynthetic activity. Provides standardized water chemistry profile...
Salamander biofluorescence photography under blue excitation (Amphibia)
Standardized photography protocol using blue light excitation to capture biofluorescence in salamanders under controlled conditions with specific camera settings and positioning.
body condition index (Ambystomatidae)
Calculation of body condition metrics (mass/SVL³) and derived fitness proxies including condition-weighted reproductive opportunities for males and estimated egg production for females based on body s...
Ceramic tile algal biomass estimation
Use of ceramic tiles as standardized substrates for measuring benthic algal biomass via chlorophyll-a extraction and fluorometry. Includes grazed vs ungrazed comparisons to assess herbivory effects.
Ecosystem nutrient uptake measurement (Amphibia)
Nutrient addition experiments in benthic substrates and sedge biofilms to measure ecosystem demand through exponential decay models. Measures uptake capacity of different pond compartments.
Stream Solute Workshop method
Tracer Additions for Spiraling Curve Characterization method involving simultaneous injection of water tracer and nutrients with downstream breakthrough curve monitoring to quantify reach-scale nutrie...