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Salamander biofluorescence photography under blue excitation (Amphibia)
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Description
Standardized photography protocol using blue light excitation to capture biofluorescence in salamanders under controlled conditions with specific camera settings and positioning.
Typical Equipment
- 500mm long-pass lens from Nightsea
- Sony a6400 mirrorless camera
- Nightsea Xite Flashlight
- 8x10x20 cm clear plastic box
- fiberglass sheet
- black plastic sheet
- darkroom cloth
Output Measurements
- fluorescence brightness values
- grayscale intensity measurements
Papers Using This Protocol (5)
student_paper
The effects of natural sun exposure on the intensity and distribution of salamander biofluorescence
student_paper
The effects of natural sun exposure on the intensity and distribution of salamander biofluorescence
student_paper
Biofluorescence in Arizona Tiger Salamanders (Ambystoma mavortium nebulsoum) as an indicator of sexual readiness
student_paper
Changes in the biofluorescence of Ambystoma mavortium nebulosum with age
student_paper