Protocols

Genomic sequencing and demographic modeling using GADMA2 to estimate historical gene flow patterns and migration rates between populations across elevational gradients.

Extraction and quantification of desulfated glucosinolates from plant leaves using high-performance liquid chromatography with diode array and charged aerosol detection. Follows established desulfatio...

Construction of Integral Projection Models using vital rate data to project population persistence under different climate scenarios and estimate stochastic population growth rates.

Time-calibrated phylogenetic analysis using multiple genes and both maximum likelihood and Bayesian approaches to estimate evolutionary relationships and timing of trait evolution.

Sanger sequencing of BCMA3 alleles from natural accessions combined with Nanopore long-read genome assembly to identify structural genetic variants affecting glucosinolate biosynthesis.

Statistical analysis of flower constancy using Bateman's index and modified Cindex with randomization tests to determine whether butterflies exhibit constant foraging behavior.

Conditional Neutrality-Antagonistic Pleiotropy analysis estimates realized selection coefficients and uses permutation tests to identify genome-wide patterns of fitness trade-offs across environments ...

Chromosome-specific BAC contigs of Arabidopsis thaliana were used as painting probes to analyze mitotic and meiotic chromosomes of C. cordifolia. Fluorescent signals were detected and analyzed to dete...

Estimation of genetic parameters including heritability and genetic variance-covariance matrices using generalized linear mixed models with Bayesian MCMC methods.

Used phylogenetic generalized least squares (PGLS) regression and phylogenetic ANOVA to test relationships between acoustic call frequencies, body size, and habitat while controlling for evolutionary ...

454 sequencing to characterize genetic diversity at the DRB Class II locus in montane voles.