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HPLC glucosinolate analysis

Subcategory: chemical analysis
Standard reference: Prasad et al. (2012) and Keith and Mitchell-Olds (2017)
Papers: 6 | Mentions: 8

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Description

Extraction and quantification of desulfated glucosinolates from plant leaves using high-performance liquid chromatography with diode array and charged aerosol detection. Follows established desulfation protocols for GSL analysis.

Typical Equipment

  • screw-cap microcentrifuge tubes
  • 70% MeOH
  • high-performance liquid chromatography (HPLC)
  • diode array detector (DAD)
  • charged aerosol detector (CAD)

Output Measurements

  • glucosinolate concentrations
  • GSL structural identification

Papers Using This Protocol (6)

article

Novel host unmasks heritable variation in plant preference within an insect population

2022Evolutionusing
article

Ecological factors influence balancing selection on leaf chemical profiles of a wildflower

2021Nature Ecology & Evolutionusing
article

Experience may outweigh cue similarity in maintaining a persistent host plant-based evolutionary trap.

2020Ecological Monographs. doi 10.1002/ecm.1412using
article

Heritable plant phenotypes track light and herbivory levels at fine spatial scales

2018Oecologiausing
article

Aversion and attraction to harmful plant secondary compounds jointly shape the foraging ecology of a specialist herbivore

2016Ecology and Evolutionusing
student_paper

<i>Scaptomyza nigrita</i> herbivory and inducible glucosinolates in <i>Cardamine cordifolia</i>

2010using