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Comparative chromosome painting (Brassicaceae)

Subcategory: cytogenetic
Standard reference: Lysak and Mandáková (2013)
Papers: 2 | Mentions: 3

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Description

Chromosome-specific BAC contigs of Arabidopsis thaliana were used as painting probes to analyze mitotic and meiotic chromosomes of C. cordifolia. Fluorescent signals were detected and analyzed to determine chromosome structure and evolutionary relationships.

Typical Equipment

  • RNase
  • pepsin
  • biotin-dUTP
  • digoxigenin-dUTP
  • Cy3-dUTP
  • DAPI
  • Olympus BX-51 microscope

Output Measurements

  • chromosome structure
  • translocation events
  • karyotype composition

Papers Using This Protocol (2)

article

Young inversion with multiple linked QTLs under selection in a hybrid zone

2017Nature Ecology and Evolutionusing
article

How diploidization turned a tetraploid into a pseudotriploid

2016American Journal of Botanyusing