Protocols

Integrated groundwater flow and reactive transport modeling using PHT-USG coupled with MODFLOW-USG for geochemical reactions and PHREEQC for reaction modeling. Includes parameter estimation using PEST...

Systematic collection of sediment samples from floodplain sites at regular depth intervals followed by DNA extraction and metagenomic sequencing to characterize microbial communities.

Integrates multiple spatial data layers including lidar, airborne geophysics, hyperspectral remote sensing, and satellite data to characterize hillslope properties. Computes average values and percent...

Time-lapse electrical resistivity imaging using dipole-dipole array configuration to map subsurface electrical conductivity patterns and estimate soil moisture distribution across the hillslope.

Comprehensive deployment of precipitation, atmospheric, and surface energy budget sensors across elevation and topographic gradients in complex terrain. Network includes core sites with full instrumen...

Systematic collection of snowpack samples across elevation gradients using snow pits dug at 10 cm increments to obtain SWE-weighted composite isotopic signatures representative of accumulated snowpack...

Collection and isotopic analysis of xylem and soil water to determine plant water sources using δ18O and δ2H signatures combined with Bayesian mixing model to quantify proportional contributions from ...

Sampling of 3H and 4He environmental tracers from screened groundwater wells to constrain groundwater age distributions and validate numerical model predictions of mixing between modern and pre-modern...

Uses stable isotope ratios (δ²H and δ¹⁸O) of precipitation and streamflow to quantify the relative contributions of snow versus rain to streamflow and evapotranspiration through endmember mixing and s...

Random Forest regression to predict ecosystem drought sensitivity from hydrological and topographic variables. Uses bootstrapped subsampling with multiple regression trees to identify key predictive v...

Standard metagenomics pipeline involving assembly with MEGAHIT, binning with multiple tools, dereplication, and taxonomic classification using GTDB-tk.

Deionized water extraction of sediments following Gregorich and Carter (2007) to measure dissolved nutrients and organic carbon. Designed to minimize physical disruption and reflect carbon and nutrien...

A comprehensive statistical framework for quality assurance and quality control of meteorological time series data involving preliminary data exploration, quality assessment with outlier detection, an...

Sequential extraction and ICP-MS analysis of soil pools and plant tissues to trace nutrient sources using 87Sr/86Sr ratios. Includes mixing model calculations to quantify proportional contributions fr...