Protocols

Standard enzyme electrophoresis technique used to determine genotypes at the 6PGD-2 locus for parentage assignment. Distinguishes between homozygous medium (MM) and homozygous slow (SS) allelic varian...

PCR amplification of microsatellite loci followed by capillary electrophoresis for allele sizing and polymorphism assessment.

Generation of null models that maintain interaction totals per species while randomizing interaction patterns, followed by permutation tests to assess significance of observed network metrics relative...

Long-term monitoring of plant morphological traits across permanent transects spanning a hybrid zone between two Ipomopsis species along an elevational gradient, with cline fitting to quantify trait g...

Weekly assessment of fungal rust infection using a 6-class ordinal scale from absent (0) to 75-100% infected (5), with infection location recorded.

Systematic collection of seeds from plants along established transects for genetic analysis. Seeds collected within defined distances of sampling points and pooled by maternal plant.

Statistical analysis using zero-inflated negative binomial generalized linear mixed models to account for excess zeros in ecological count data, specifically pollen grain counts.

Non-destructive estimation of leaf chlorophyll concentration using reflectance spectroscopy and vegetation indices.

Development of deterministic and stochastic population models incorporating empirical estimates of selection, heritability, and phenotypic plasticity to project population persistence under climate ch...

Replicated split-plot design with snowmelt manipulated at plot level and precipitation manipulated at subplot level.