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Microsatellite genotyping (Polemoniaceae)

Subcategory: molecular
Standard reference: Bachmann, 1994
Papers: 3 | Mentions: 3

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Description

PCR amplification of microsatellite loci followed by capillary electrophoresis for allele sizing and polymorphism assessment.

Typical Equipment

  • Eppendorf Mastercycler thermocyclers
  • ABI 3730xl sequencer
  • ROX 500 size standard
  • GeneMarker software

Output Measurements

  • allele sizes
  • heterozygosity
  • number of alleles per locus

Papers Using This Protocol (3)

article

Microsatellite loci in <i>Ipomopsis aggregata</i> (Polemoniaceae) and cross-species applicability for ecological genetics studies

2012American Journal of Botanyusing
article

Genetic and morphological patterns show variation in frequency of hybrids between <i>Ipomopsis</i> (Polemoneaceae) zones of sympatry

2009Heredityusing
article

Leaf physiology reflects environmental differences and cytoplasmic background in <i>Ipomopsis</i> hybrids

2007American Journal of Botanyusing