Protocols
13 protocols
soil respiration measurement
Continuous measurement of soil CO2 concentrations at multiple depths using automated sensors to calculate soil CO2 fluxes via gradient approach. Includes soil temperature and moisture monitoring for e...
Loss-on-ignition method
Systematic excavation of 1-meter soil pits with depth-stratified sampling at 10 cm intervals for soil organic carbon, nitrogen, and dissolved organic carbon analysis. Includes soil profile description...
Factorial field experiment
Factorial experiment crossing nitrogen addition treatments with dominant species removal treatments in replicated plots to test community responses to multiple global change drivers. Includes both sel...
Leaf pack method
Standard litterbag method deployed across elevation gradient to study litter decomposition rates and microbial succession. Involves placing known masses of litter in mesh bags and retrieving over time...
Chloroform fumigation extraction
Standard chloroform fumigation direct extraction technique for measuring soil microbial biomass, combined with soil chemistry analysis and enzyme activity assays.
GIS spatial data extraction (Plantae)
Integration of field GPS coordinates with multiple spatial datasets (elevation, slope, land cover, climate) to characterize environmental conditions at population locations. Uses established spatial d...
Meta-analysis with log-response ratios
Comprehensive search of Web of Science databases for manipulative experiments on terrestrial carbon cycling responses to global change drivers, followed by systematic screening, data extraction, and q...
FTIR with ATR accessory (Pinaceae)
Fourier Transform Infrared spectroscopy analysis of ground needle samples to quantify functional group concentrations including aromatics, ethers, amides, and carbonyls. Uses attenuated-total-reflecti...
Systematic review of microbial macroecology literature
A comprehensive systematic review using database searches, citation network expansion, and vote counting to assess evidence for macroecological rules in microorganisms across multiple taxonomic groups...
soil enzyme activity assay
Measurement of six different soil extracellular enzyme activities using microplate spectrophotometry to understand microbial activity and nutrient cycling processes in response to plant removal treatm...
Repeatability analysis
Statistical analysis using subset of main data to assess repeatability of measurements.
Water use efficiency analysis (Plantae)
R script analysis of sex differences in integrated water use efficiency and population operational sex ratios in Valeriana edulis.
census (Plantae)
Population counting and demographic data collection of plant species.