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soil enzyme activity assay

Subcategory: enzymatic
Standard reference: Saiya-Cork and Sinsabaugh (2002)
Papers: 2 | Mentions: 3

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Description

Measurement of six different soil extracellular enzyme activities using microplate spectrophotometry to understand microbial activity and nutrient cycling processes in response to plant removal treatments.

Typical Equipment

  • Synergy HT microplate spectrophotometer
  • sodium acetate buffer

Output Measurements

  • α-glucosidase activity
  • β-glucosidase activity
  • cellobiohydrolase activity
  • β-Xylosidase activity
  • β-N-acetylglucosamini-dase activity
  • acid phosphatase activity

Papers Using This Protocol (2)

article

Soil signals of key mechanisms driving greater protection of organic carbon under aspen compared to spruce forests in a North American montane ecosystem

2025Biogeosciencesusing
article

Plant removal across an elevational gradient marginally reduces rates, substantially reduces variation in mineralization

2022Ecologyusing