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Protocols

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9 protocols

observationalstandardized

mark-recapture

Beetles were captured using speed nets, marked with tungsten needle dot codes on elytra, and released for recapture monitoring to estimate population sizes and dispersal patterns. Elytral condition wa...

demographic monitoring27 paperslong handled speed net, plastic containers for transport...
measurementstandardized

LI-COR gas exchange measurements (Asteraceae)

Comprehensive measurement of leaf morphological, physiological, and chemical traits including stomatal conductance, leaf mass per area, nitrogen content, and spectral properties to characterize plant ...

plant physiology5 paperssteady-state porometer (LI-COR, LI-1600M), flatbed scanner (Canon, LIDE 220)...
measurementstandardized

Butterfly fecundity and longevity monitoring (Nymphalidae)

Daily monitoring of individual female butterflies including artificial feeding with sucrose solution and daily egg collection. Measures lifetime reproductive output and adult lifespan under controlled...

demographic4 papersHamilton syringe, 25% v/v sucrose water...
analyticalstandardized

microsatellite analysis (Animalia)

Pooled whole genome sequencing approach using DNA from multiple individuals per population to identify SNPs associated with environmental variables and population differentiation patterns.

genomic4 paperscollection containers, ethanol...
analytical

GIS spatial data extraction (Plantae)

Integration of field GPS coordinates with multiple spatial datasets (elevation, slope, land cover, climate) to characterize environmental conditions at population locations. Uses established spatial d...

geospatial analysis3 papersGPX Editor software, R statistical software...
measurement

Subsurface temperature logging

Long-term monitoring of subsurface temperatures in pika habitat using buried data loggers to characterize thermal stress and microclimate conditions.

microclimate monitoring3 papersHOBO temperature data loggers, Onset U10-003
analyticalstandardized

multistate capture-mark-recapture models (Animalia)

Statistical modeling of capture-recapture data to estimate seasonal survival probabilities across age classes using program MARK with model selection via AICc.

demographic analysis2 papersprogram MARK software, R statistical software
observational

census (Plantae)

Population counting and demographic data collection of plant species.

0 papers
sampling

demographic trap records (Animalia)

Trapping animals to record age, sex, locality, reproductive status, location changes, fate, and genetic information. Data collected across 37 years at 12 sites.

0 papers