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microsatellite analysis (Animalia)

Subcategory: genomic
Standard reference: Miller et al. 1988; Schlötterer 1998
Papers: 4 | Mentions: 15

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Description

Pooled whole genome sequencing approach using DNA from multiple individuals per population to identify SNPs associated with environmental variables and population differentiation patterns.

Typical Equipment

  • collection containers
  • ethanol
  • CTAB solution
  • Qubit
  • Illumina HiSeq3000
  • FASTQC
  • TRIMMOMATIC
  • BWA-MEM
  • SAMTOOLS
  • GATK

Output Measurements

  • SNP frequencies
  • FST values
  • population genetic statistics

Papers Using This Protocol (4)

article

Evolution and genomic basis of the plant penetrating ovipositor: a key morphological trait in herbivorous Drosophilidae

2022Proceedings of the Royal Society Busing
article

Cold adaptation drives population genomic divergence in the ecological specialist, <i> Drosophila montana </i>

2021Molecular Ecologyusing
article

Phylogeographic patterns in <i>Drosophila montana</i>

2007Molecular Ecologyusing
article

Microsatellite-based species identification method for <i>Drosophila virilis</i> group species

2007Hereditasintroducing