Protocols
15 protocols
reciprocal transplant experiment
Multi-site common garden experiment using controlled crosses to partition genetic and environmental variance in vegetative and floral traits across a hybrid zone. Seeds from multiple genetic families ...
Road dust impact on floral longevity experiment
Paired experimental design comparing floral development and longevity between plants receiving daily road dust applications versus undusted controls, with detailed tracking of flower developmental sta...
Silicon tubing volatile collection (Polemoniaceae)
Volatile organic compounds were sampled from single flowers using dynamic headspace sampling, then analyzed with thermal desorption gas chromatography-mass spectrometry to identify and quantify floral...
reflectance spectrophotometry
Standardized measurement of floral reflectance spectra using fiber optic probe positioned at fixed distance and angle from flower surface. Spectra collected across 300-700 nm range for input into visu...
Integral Projection Model
Size-structured population model that incorporates climate effects on vital rates to project population dynamics and calculate population growth rates. Uses continuous size distributions and matrix pr...
Phenotypic selection analysis
Quantified natural selection on floral traits by measuring relationships between trait values, pollinator visitation rates, and reproductive success. Calculated standardized selection differentials to...
Artificial flower UV exposure selection experiment
Controlled experiment using artificial flowers with discrete bullseye phenotypes exposed to UV-present vs UV-absent treatments to test UV-mediated selection on floral traits. Pollen viability measured...
focal animal sampling
Standardized pellet count method for estimating deer density using systematic transect surveys with random placement. Identifies scat to species level where possible to distinguish herbivore types usi...
Camera trap hummingbird visitation monitoring (Aves)
Time-lapse photography of individual flowers using motion-activated cameras to capture and quantify hummingbird feeding visits. Combined with machine learning image processing to automate detection.
Specific leaf area (SLA) measurement
Standard protocol for measuring leaf traits by scanning fresh leaves, counting trichomes using ImageJ, then drying and weighing leaves to calculate specific leaf area. Uses ImageJ software for digital...
Starch gel electrophoresis (Polemoniaceae)
Standard enzyme electrophoresis technique used to determine genotypes at the 6PGD-2 locus for parentage assignment. Distinguishes between homozygous medium (MM) and homozygous slow (SS) allelic varian...
focal animal sampling (Cervidae)
Controlled experiment using feeding stations paired with predator scent cues to test antipredator behavioral responses. Individual feeding times are recorded to quantify vigilance behavior in response...
Integral projection model (Brassicaceae)
Construction of Integral Projection Models using vital rate data to project population persistence under different climate scenarios and estimate stochastic population growth rates.
herbivore exclosure experiment (Plantae)
Factorial experiment using wire mesh and chicken wire fencing to exclude mammalian herbivores (ungulates and gophers) from transplanted alpine plants across elevational gradients. Plants monitored for...
Plant fitness monitoring in field experiment (Plantae)
Repeated measurements of plant morphological traits (tillers, leaves, height) and survival tracking to assess fitness responses to experimental treatments.