Protocols

Uses full-waveform LiDAR returns processed through adaptive deconvolution and individual tree detection algorithms to map forest structure metrics including tree density, height distributions, and bas...

Wood samples are sectioned into sub-annual increments and cellulose is extracted for oxygen and carbon isotope analysis to assess seasonal water sources and water use efficiency in trees.

Time-lapse UAV surveys using RGB and multispectral cameras to monitor vegetation indices (NDVI, GCC) and plant height changes across a hillslope transect during growing seasons.

Standard increment boring technique to extract tree cores for measuring annual ring widths, then calculating basal area increment over specific time periods to quantify stem growth.

Standard chloroform fumigation direct extraction technique for measuring soil microbial biomass, combined with soil chemistry analysis and enzyme activity assays.

Standard Illumina library preparation using Nextera XT kit followed by paired-end sequencing on HiSeq 2500 platform to generate comprehensive metagenomic datasets from phylloplane microbial communitie...

Autonomous sampling system using osmotic pumps to continuously collect water samples over extended periods for chemical analysis. Tubing inlets placed at different depths collect time-integrated sampl...

Continuous measurement of tree water transport using thermal dissipation sensors with temperature measurements at multiple depths in sapwood to calculate sap velocity via heat pulse methodology.

Data acquired using a Riegl Q1560 dual-channel LiDAR system mounted on a Piper Navajo aircraft. Survey complied with USGS QL1 standard with point density more than 8 pulses per m2.

Cellulose extraction using Brendel method followed by pyrolysis at 1400°C and isotopic ratio measurement by GC-IRMS.

National Ecological Observatory Network Airborne Observation Platform flights acquiring LiDAR, imaging spectroscopy, and high-resolution camera imagery.

Measurement of hydrogen and oxygen isotope ratios using Off-Axis Integrated Cavity Output Spectrometer with auto-sampler and heated injector block. Results reported in δ-notation relative to Vienna St...