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Illumina paired-end sequencing
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Description
Standard Illumina library preparation using Nextera XT kit followed by paired-end sequencing on HiSeq 2500 platform to generate comprehensive metagenomic datasets from phylloplane microbial communities.
Typical Equipment
- Nextera XT kit
- SPRI beads
- Beckman Coulter
- LightCycler 480 real-time PCR instrument
- HiSeq 2500
Output Measurements
- 150 bp paired-end metagenomic reads
Papers Using This Protocol (5)
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Host species and geographic location shape microbial diversity and functional potential in the conifer needle microbiome
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Virus diversity and activity is driven by snowmelt and host dynamics in a high-altitude watershed soil ecosystem
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Meanders as a scaling motif for understanding of floodplain soil microbiome and biogeochemical potential at the watershed scale
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Meanders as a scaling motif for understanding of floodplain soil microbiome and biogeochemical potential at the watershed scale
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