Protocols

Infrared heaters suspended above experimental plots to simulate warming effects on montane vegetation communities across moisture gradients. Heating power adjusted over time to maintain consistent war...

29-year experimental warming using overhead infrared radiators to increase soil temperature by 2°C in subalpine grassland plots. Heat flux of 22 W/m² applied continuously from 1991-2019.

Factorial greenhouse experiment testing effects of soil inoculum source and sterilization on alpine grass growth, traits, and fungal colonization. Plants grown in standardized sand-inoculum mixtures w...

A controlled field experiment transplanting grass species across elevational gradients with manipulated fungal soil communities to test effects of plant-fungi mismatches on plant performance during cl...

Field experiment testing seed germination of different genotypes/treatments in natural microhabitats of related species to assess niche requirements and differentiation.

Systematic presentation of conspecific, heterospecific competitor, and control songs to territorial males followed by standardized behavioral observation and scoring. Uses randomized treatment order w...

Seeds were treated with Benomyl fungicide at 4°C to remove endophytic fungi, creating endophyte-free plants for comparison with naturally endophyte-positive and endophyte-negative plants.

Controlled temperature treatments from 15-65°C applied to detached leaves for 1 hour, with temperature ramped at 2°C/hour. Used to determine lethal temperature thresholds via electrolyte leakage measu...