Electrolyte leakage assay (Asteraceae)

Subcategory: physiological stress testing

Papers: 2 | Mentions: 2

Local Knowledge Graph (17 entities)

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Description

Controlled temperature treatments from 15-65°C applied to detached leaves for 1 hour, with temperature ramped at 2°C/hour. Used to determine lethal temperature thresholds via electrolyte leakage measurements.

Typical Equipment

modified picnic cooler
heat exchanger
circulating water bath
60% ethylene glycol solution
number 1 cork borer
Cole-Parmer Chemcadet pH/conductivity meter

Output Measurements

LT50 values
electrolyte leakage percentages

Papers Using This Protocol (2)

student_paper

The Effect of Ant Colony Proximity on Membracid Survivorship

2005using

article

High-temperature tolerance of <i>Artemisia tridentata</i> and <i>Potentilla gracilis</i> under a climate change manipulation

1996Oecologiausing