Protocols
17 protocols
Hemacytometer pollen counting
Collection and microscopic analysis of pollen grains deposited on wildflower stigmas using pink fuchsin gel mounting and reference collection for identification. Quantifies both conspecific and hetero...
correlation analysis
Statistical analysis using mixed effects models to evaluate relationships between plant clumping treatment and temperature responses while accounting for random effects of individual plants and sampli...
Silicon tubing volatile collection (Polemoniaceae)
Volatile organic compounds were sampled from single flowers using dynamic headspace sampling, then analyzed with thermal desorption gas chromatography-mass spectrometry to identify and quantify floral...
Carbon isotope discrimination analysis
Measurement of water-use efficiency differences between plant sexes using leaf carbon isotope ratios (δ13C) to understand mechanistic basis of sex-specific climate responses.
Phenotypic selection analysis
Quantified natural selection on floral traits by measuring relationships between trait values, pollinator visitation rates, and reproductive success. Calculated standardized selection differentials to...
Nestedness analysis
Pollen-transport interactions were analyzed using network metrics including weighted connectance, linkage density, weighted nestedness, specialization and robustness calculated with R package bipartit...
Path analysis
Statistical approach to test causal pathways between herbivory, plant characteristics, and reproductive output using multiple linked regression models with model selection based on information criteri...
Whittaker's dissimilarity index
Calculation of interaction turnover between plant-pollinator networks at different sites by comparing shared taxa and their interaction frequencies. Produces metrics of rewiring occurrence and interac...
degree-day models
Calculation of cumulative growing degree days from weather station data using species-specific base temperatures, correlated with biological events through linear regression to assess temperature-depe...
iNEXT rarefaction
Statistical analysis of pollinator diversity using Shannon Diversity Index with ANOVA and post-hoc testing to compare plant visitation diversity between pollinator groups.
Dichotomous key construction (Onagraceae)
A systematic approach to creating identification keys by compiling species lists from authoritative sources, gathering morphological descriptions, and structuring binary choices that eliminate possibi...
Structural equation modeling (Polemoniaceae)
Path analysis using structural equation modeling to test competing hypotheses about causal relationships between nectar traits, robbing, pollination, and fitness.
ICP-AES elemental analysis
Acid digestion of plant and soil samples followed by Inductively Coupled Plasma-Atomic Emission Spectrometry to quantify metal concentrations in tissues. Standard laboratory protocol for elemental ana...
Lupine alkaloid extraction and GC analysis (Fabaceae)
Comprehensive method for extracting quinolizidine and piperidine alkaloids from plant tissues using acid-base extraction followed by GC/FID and GC/MS analysis for identification and quantification.
Multi-scale plant-pollinator network aggregation
A method for constructing plant-pollinator interaction networks at multiple temporal scales from temporally-explicit field observations. Networks are aggregated from daily observations to weekly, mont...
Temperature-behavior correlation analysis
Linear regression analyses testing relationships between daily temperature deviations and hummingbird visitation rates. Multiple analytical approaches including yearly regressions, temperature binning...
Transcriptomic analysis (Animalia)
Transcriptomic analysis of whirling disease-resistant rainbow trout to understand gene expression patterns related to resistance.