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Hemacytometer pollen counting
Local Knowledge Graph (41 entities)
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Description
Collection and microscopic analysis of pollen grains deposited on wildflower stigmas using pink fuchsin gel mounting and reference collection for identification. Quantifies both conspecific and heterospecific pollen deposition.
Typical Equipment
- tweezers
- small eppendorf tubes
- cooler bag
- labels
- pink fuchsin gel
- glass microscope slides
- hot plate
- square microscope glass coverslips
- pencil with eraser
- marker
- compound microscope
- pollen reference collection from RMBL herbarium
Output Measurements
- conspecific pollen grain counts
- heterospecific pollen grain counts
- proportion of conspecific pollen grains
Papers Using This Protocol (51)
student_paper
Observing the effects of a nectar-inhabiting bacterium, Pantoea sp., and nectar robbing on male fitness in Ipomopsis aggregata
thesis
Hydrologic connectivity shapes watershed response to climate variability from local to global scales
student_paper
Fit or Unlit: using quantum dots to investigate the effects of a floricolous yeast and nectar robbing on male fitness in Ipomopsis aggregata
student_paper
Fit or Unlit: using quantum dots to investigate the effects of a floricolous yeast and nectar robbing on male fitness in Ipomopsis aggregata
student_paper
How does early snowmelt affect pollen deposition on spring wildflowers?
student_paper
Effects of clumping of <i> Delphinium barbeyi </i> on pollination
student_paper
Effects of Floral Abundance, Pollinator Interactions, and Floral Morphology on Stigmatic Pollen Deposition
student_paper
Variation of Pollen Mass Across Species, Habitat Types, and Time
article
Open-top warming chambers reduce animal pollination of two subalpine herbs
student_paper
The Effects of Exclusion Bags and Open-Top Chambers on Pollinator Activity
student_paper
The Effects of Climate-Driven Changes in Co-flowering between <i>Linum lewisii</i> and <i>Potentilla pulcherrima</i> on Pollinator Services
article
Effects of road dust on the pollination and reproduction of wildflowers
student_paper
Do the differing pollen foraging strategies of <i>Megachile sp.</i> and <i>Bombus spp.</i> result in differing pollen removal and deposition rates in <i>Lupinus bakeri</i>?
article
Facilitated exploitation of pollination mutualisms: fitness consequences for plants
article
Drought, pollen and nectar availability, and pollination success
student_paper
Sex ratio and reproductive success along elevational gradients of gynodioecious populations of <i>Geranium richardsonii</i>
student_paper
Assessing pollinators’ contribution to the reproductive success of <i>Mertensia ciliata</i>
article
Heterospecific pollen deposition in <i>Delphinium barbeyi</i>: linking stigmatic pollen loads to reproductive output in the field
article
An altitudinal cline in UV floral pattern corresponds with a behavioral change of a generalist pollinator assemblage
article
Selection of trait combinations through bee and fly visitation to flowers of <i>Polemonium foliosissimum</i>
student_paper
Consequences of Pollination Neighborhood Composition and Pollinator Communities
student_paper
Effects of road proximity on pollinator visitation and reproductive success of Ipomopsis aggregate.
student_paper
The Impact of Primary and Secondary Robbing on Hummingbird Pollination.
student_paper
Correlating road dust with reproduction of Ipomopsis aggregate.
student_paper
Quantifying the effects of pollen load and heterospecific pollen ratio on the species <i>Delphinium barbeyi</i> (Ranunculaceae)
student_paper
How does altered phenology affect pollination and reproduction of <i>Delphinium nuttallianum</i>?
student_paper
Comparing <i>Bombus</i> pollinator efficacies in <i>Delphinium barbeyi</i> (Ranunculaceae) reproductive success
student_paper
The effects of road dust on pollination and reproduction of the native wildflower species, <i>Delphinium nuttallianum</i>
article
When resources don't rescue: flowering phenology and species interactions affect compensation to herbivory in <i>Ipomopsis aggregata</i>
student_paper
The effects of phenology on <i>Delphinium nuttallianum</i>
article
Additive effects of herbivory, nectar robbing and seed predation on male and female fitness components of the host plant <i>Ipomopsis aggregata</i>
student_paper
Nectar and pollen rewards of invasive plants and their native congeners
student_paper
Effects of multi-species interactions in gynodioecious populations of <i>Polemonium foliosissimum</i>
student_paper
Effects of quantity and distribution of pollen on fertilization in the gynodioecious species <i>Geranium richardsonii</i>
article
Realized tolerance to nectar robbing: compensation to floral enemies in <i>Ipomopsis aggregata</i>
student_paper
Does road dust affect reproduction of <i>Delphinium nelsonii</i> and <i>Ipomopsis aggregata</i>
article
Mechanisms of tolerance to floral larceny in two animal-pollinated wildflowers, <i>Polemonium viscosum</i> and <i>Ipomopsis aggregata</i>
student_paper
The effect of sampling effort on species richness estimates of flower visitors
student_paper
The Effects of Soil Nitrogen Availability on Plant Reproduction and Solitary Bee Behavior
student_paper
Nectar robbing in Ipomopsis aggregata: does high nectar production confer tolerance?
student_paper
Effects of Interspecific Pollen Transfer (IPT) in a Specialist and a Generalist Flower
article
Temporal and spatial variation in pollination of a montane herb: a seven-year study
article
The dual role of floral traits: pollinator attraction and plant defense
article
Direct and indirect effects of pollinators and seed predators to selection on plant and floral traits
article
Rescue of stranded pollen grains by secondary transfer
article
Linking pollinator visitation rate and pollen receipt
article
Rescue of stranded pollen grains by secondary transfer
article
Impact of nectar robbing on estimates of pollen flow: conceptual predictions and emperical outcomes
article
Predicting patterns of mating and potential hybridization from pollinator behavior
student_paper
The effects of ungulate herbivory and nutrient variation on pollen reciept in Ipomopsis aggregata
student_paper
