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Hemacytometer pollen counting

Subcategory: microscopy
Standard reference: Kearns and Inouye 1993
Papers: 49 | Mentions: 55

Local Knowledge Graph (41 entities)

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Description

Collection and microscopic analysis of pollen grains deposited on wildflower stigmas using pink fuchsin gel mounting and reference collection for identification. Quantifies both conspecific and heterospecific pollen deposition.

Typical Equipment

  • tweezers
  • small eppendorf tubes
  • cooler bag
  • labels
  • pink fuchsin gel
  • glass microscope slides
  • hot plate
  • square microscope glass coverslips
  • pencil with eraser
  • marker
  • compound microscope
  • pollen reference collection from RMBL herbarium

Output Measurements

  • conspecific pollen grain counts
  • heterospecific pollen grain counts
  • proportion of conspecific pollen grains

Papers Using This Protocol (51)

student_paper

Observing the effects of a nectar-inhabiting bacterium, Pantoea sp., and nectar robbing on male fitness in Ipomopsis aggregata

2025using
thesis

Hydrologic connectivity shapes watershed response to climate variability from local to global scales

2025using
student_paper

Fit or Unlit: using quantum dots to investigate the effects of a floricolous yeast and nectar robbing on male fitness in Ipomopsis aggregata

2024introducing
student_paper

Fit or Unlit: using quantum dots to investigate the effects of a floricolous yeast and nectar robbing on male fitness in Ipomopsis aggregata

2024using
student_paper

How does early snowmelt affect pollen deposition on spring wildflowers?

2024using
student_paper

Effects of clumping of <i> Delphinium barbeyi </i> on pollination

2022using
student_paper

Effects of Floral Abundance, Pollinator Interactions, and Floral Morphology on Stigmatic Pollen Deposition

2022using
student_paper

Variation of Pollen Mass Across Species, Habitat Types, and Time

2021introducing
article

Open-top warming chambers reduce animal pollination of two subalpine herbs

2021Journal of Pollination Ecologyusing
student_paper

The Effects of Exclusion Bags and Open-Top Chambers on Pollinator Activity

2019Student paperusing
student_paper

The Effects of Climate-Driven Changes in Co-flowering between <i>Linum lewisii</i> and <i>Potentilla pulcherrima</i> on Pollinator Services

2018using
article

Effects of road dust on the pollination and reproduction of wildflowers

2017International Journal of Plant Sciencesusing
student_paper

Do the differing pollen foraging strategies of <i>Megachile sp.</i> and <i>Bombus spp.</i> result in differing pollen removal and deposition rates in <i>Lupinus bakeri</i>?

2017using
article

Facilitated exploitation of pollination mutualisms: fitness consequences for plants

2017Journal of Ecologyusing
article

Drought, pollen and nectar availability, and pollination success

2016Ecologyusing
student_paper

Sex ratio and reproductive success along elevational gradients of gynodioecious populations of <i>Geranium richardsonii</i>

2016using
student_paper

Assessing pollinators’ contribution to the reproductive success of <i>Mertensia ciliata</i>

2016using
article

Heterospecific pollen deposition in <i>Delphinium barbeyi</i>: linking stigmatic pollen loads to reproductive output in the field

2016Annals of Botanyusing
article

An altitudinal cline in UV floral pattern corresponds with a behavioral change of a generalist pollinator assemblage

2015Ecologyusing
article

Selection of trait combinations through bee and fly visitation to flowers of <i>Polemonium foliosissimum</i>

2014Journal of Evolutionary Biologyusing
student_paper

Consequences of Pollination Neighborhood Composition and Pollinator Communities

2014using
student_paper

Effects of road proximity on pollinator visitation and reproductive success of Ipomopsis aggregate.

2014using
student_paper

The Impact of Primary and Secondary Robbing on Hummingbird Pollination.

2014using
student_paper

Correlating road dust with reproduction of Ipomopsis aggregate.

2014using
student_paper

Quantifying the effects of pollen load and heterospecific pollen ratio on the species <i>Delphinium barbeyi</i> (Ranunculaceae)

2013introducing
student_paper

How does altered phenology affect pollination and reproduction of <i>Delphinium nuttallianum</i>?

2013using
student_paper

Comparing <i>Bombus</i> pollinator efficacies in <i>Delphinium barbeyi</i> (Ranunculaceae) reproductive success

2013using
student_paper

The effects of road dust on pollination and reproduction of the native wildflower species, <i>Delphinium nuttallianum</i>

2013using
article

When resources don't rescue: flowering phenology and species interactions affect compensation to herbivory in <i>Ipomopsis aggregata</i>

2012Oikosusing
student_paper

The effects of phenology on <i>Delphinium nuttallianum</i>

2011using
article

Additive effects of herbivory, nectar robbing and seed predation on male and female fitness components of the host plant <i>Ipomopsis aggregata</i>

2011Oecologiausing
student_paper

Nectar and pollen rewards of invasive plants and their native congeners

2010using
student_paper

Effects of multi-species interactions in gynodioecious populations of <i>Polemonium foliosissimum</i>

2010using
student_paper

Effects of quantity and distribution of pollen on fertilization in the gynodioecious species <i>Geranium richardsonii</i>

2009using
article

Realized tolerance to nectar robbing: compensation to floral enemies in <i>Ipomopsis aggregata</i>

2009Annals of Botanyusing
student_paper

Does road dust affect reproduction of <i>Delphinium nelsonii</i> and <i>Ipomopsis aggregata</i>

2008using
article

Mechanisms of tolerance to floral larceny in two animal-pollinated wildflowers, <i>Polemonium viscosum</i> and <i>Ipomopsis aggregata</i>

2008Ecologyusing
student_paper

The effect of sampling effort on species richness estimates of flower visitors

2007using
student_paper

The Effects of Soil Nitrogen Availability on Plant Reproduction and Solitary Bee Behavior

2006using
student_paper

Nectar robbing in Ipomopsis aggregata: does high nectar production confer tolerance?

2005using
student_paper

Effects of Interspecific Pollen Transfer (IPT) in a Specialist and a Generalist Flower

2005using
article

Temporal and spatial variation in pollination of a montane herb: a seven-year study

2005Ecologyusing
article

The dual role of floral traits: pollinator attraction and plant defense

2004Ecologyusing
article

Direct and indirect effects of pollinators and seed predators to selection on plant and floral traits

2004Oikosusing
article

Rescue of stranded pollen grains by secondary transfer

2003Plant Species Biologyintroducing
article

Linking pollinator visitation rate and pollen receipt

2003American Journal of Botanyusing
article

Rescue of stranded pollen grains by secondary transfer

2003Plant Species Biologyusing
article

Impact of nectar robbing on estimates of pollen flow: conceptual predictions and emperical outcomes

2003Ecologyusing
article

Predicting patterns of mating and potential hybridization from pollinator behavior

2002American Naturalistusing
student_paper

The effects of ungulate herbivory and nutrient variation on pollen reciept in Ipomopsis aggregata

2002introducing
student_paper

The effect of hermaphrodite density and frequency at three spatial scales on the polen receipt and seed set of gynodioecious Geranium richardsonii (Geraniaceae)

2001using