Lupine alkaloid extraction and GC analysis (Fabaceae)

Subcategory: chemical analysis

Standard reference: Lee et al. (2007) - partially based on previous methods

Papers: 1 | Mentions: 3

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Description

Comprehensive method for extracting quinolizidine and piperidine alkaloids from plant tissues using acid-base extraction followed by GC/FID and GC/MS analysis for identification and quantification.

Typical Equipment

Rugged Rotator
Shimadzu GC-2010
Finnigan MAT GCQ
centrifuge

Output Measurements

alkaloid concentrations
alkaloid identification
alkaloid composition profiles

Papers Using This Protocol (1)

article

Pollen and vegetative secondary chemistry of three pollen-rewarding lupines

2019American Journal of Botanyusing