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Protocols

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8 protocols

analyticalstandardized

structure from motion

Integrates multiple spatial data layers including lidar, airborne geophysics, hyperspectral remote sensing, and satellite data to characterize hillslope properties. Computes average values and percent...

spatial data integration8 papersLidar DEM, AEM survey data...
analyticalstandardized

Endmember splitting and mixing analysis

Uses stable isotope ratios (δ²H and δ¹⁸O) of precipitation and streamflow to quantify the relative contributions of snow versus rain to streamflow and evapotranspiration through endmember mixing and s...

isotope hydrology4 papersModel 3700 automatic samplers (Teledyne ISCO, NE, USA), 2-cm mineral oil pre-filled bottles...
analyticalstandardized

Illumina paired-end sequencing

Standard Illumina library preparation using Nextera XT kit followed by paired-end sequencing on HiSeq 2500 platform to generate comprehensive metagenomic datasets from phylloplane microbial communitie...

molecular4 papersNextera XT kit, SPRI beads...
analyticalstandardized

Random Forest

Random Forest regression to predict ecosystem drought sensitivity from hydrological and topographic variables. Uses bootstrapped subsampling with multiple regression trees to identify key predictive v...

machine learning4 papersR software, randomForest package
analyticalstandardized

HydroMix

Combined use of major ion chemistry and Sr isotope signatures to identify distinct groundwater end members and quantify their contributions to stream flow using PCA-based end member mixing analysis.

geochemical source apportionment2 papersSr resin (Eichrom Technologies Inc.), miniature Teflon columns...
analyticalstandardized

Metagenomic HMM metabolic profiling

Comprehensive Hidden Markov Model analysis of metagenomic sequences to identify and quantify metabolic functions across biogeochemical pathways. Uses both standardized TIGR-FAM and custom HMM database...

molecular2 papersTIGR-FAM database, Custom HMM databases
analyticalstandardized

X-ray Absorption Near Edge Structure spectroscopy

Sulfur K-edge X-ray Absorption Near Edge Structure spectroscopy was used to determine sulfur speciation in solid samples including bedrock, soils, and sediments. Linear combination fitting was used to...

spectroscopic2 paperssynchrotron X-ray sources, XANES spectroscopy equipment...
analytical

rpS3 marker gene analysis

Taxonomic classification of microbial communities using ribosomal protein S3 (rpS3) marker genes identified through Hidden Markov Models and phylogenetic analysis.

taxonomic classification1 paperHidden Markov Models (HMM), MAFFT alignment software