Protocols
8 protocols
structure from motion
Integrates multiple spatial data layers including lidar, airborne geophysics, hyperspectral remote sensing, and satellite data to characterize hillslope properties. Computes average values and percent...
Endmember splitting and mixing analysis
Uses stable isotope ratios (δ²H and δ¹⁸O) of precipitation and streamflow to quantify the relative contributions of snow versus rain to streamflow and evapotranspiration through endmember mixing and s...
Illumina paired-end sequencing
Standard Illumina library preparation using Nextera XT kit followed by paired-end sequencing on HiSeq 2500 platform to generate comprehensive metagenomic datasets from phylloplane microbial communitie...
Random Forest
Random Forest regression to predict ecosystem drought sensitivity from hydrological and topographic variables. Uses bootstrapped subsampling with multiple regression trees to identify key predictive v...
HydroMix
Combined use of major ion chemistry and Sr isotope signatures to identify distinct groundwater end members and quantify their contributions to stream flow using PCA-based end member mixing analysis.
Metagenomic HMM metabolic profiling
Comprehensive Hidden Markov Model analysis of metagenomic sequences to identify and quantify metabolic functions across biogeochemical pathways. Uses both standardized TIGR-FAM and custom HMM database...
X-ray Absorption Near Edge Structure spectroscopy
Sulfur K-edge X-ray Absorption Near Edge Structure spectroscopy was used to determine sulfur speciation in solid samples including bedrock, soils, and sediments. Linear combination fitting was used to...
rpS3 marker gene analysis
Taxonomic classification of microbial communities using ribosomal protein S3 (rpS3) marker genes identified through Hidden Markov Models and phylogenetic analysis.
