Protocols
6 protocols
Forest inventory plot sampling
Uses full-waveform LiDAR returns processed through adaptive deconvolution and individual tree detection algorithms to map forest structure metrics including tree density, height distributions, and bas...
ARSTAN chronology development
Wood samples are sectioned into sub-annual increments and cellulose is extracted for oxygen and carbon isotope analysis to assess seasonal water sources and water use efficiency in trees.
Chloroform fumigation extraction
Standard chloroform fumigation direct extraction technique for measuring soil microbial biomass, combined with soil chemistry analysis and enzyme activity assays.
Illumina paired-end sequencing
Standard Illumina library preparation using Nextera XT kit followed by paired-end sequencing on HiSeq 2500 platform to generate comprehensive metagenomic datasets from phylloplane microbial communitie...
Cellulose isotope analysis (Plantae)
Cellulose extraction using Brendel method followed by pyrolysis at 1400°C and isotopic ratio measurement by GC-IRMS.
Stable Water Isotope Analysis
Measurement of hydrogen and oxygen isotope ratios using Off-Axis Integrated Cavity Output Spectrometer with auto-sampler and heated injector block. Results reported in δ-notation relative to Vienna St...
