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Protocols

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6 protocols

analyticalstandardized

Forest inventory plot sampling

Uses full-waveform LiDAR returns processed through adaptive deconvolution and individual tree detection algorithms to map forest structure metrics including tree density, height distributions, and bas...

remote sensing15 papersOptech Gemini discrete LiDAR sensor, waveform digitizer...
analyticalstandardized

ARSTAN chronology development

Wood samples are sectioned into sub-annual increments and cellulose is extracted for oxygen and carbon isotope analysis to assess seasonal water sources and water use efficiency in trees.

isotopic13 paperssanding equipment, scanner...
analyticalstandardized

Chloroform fumigation extraction

Standard chloroform fumigation direct extraction technique for measuring soil microbial biomass, combined with soil chemistry analysis and enzyme activity assays.

biogeochemistry4 paperschloroform fumigation extraction setup, soil chemistry analysis equipment
analyticalstandardized

Illumina paired-end sequencing

Standard Illumina library preparation using Nextera XT kit followed by paired-end sequencing on HiSeq 2500 platform to generate comprehensive metagenomic datasets from phylloplane microbial communitie...

molecular4 papersNextera XT kit, SPRI beads...
analytical

Cellulose isotope analysis (Plantae)

Cellulose extraction using Brendel method followed by pyrolysis at 1400°C and isotopic ratio measurement by GC-IRMS.

0 papers
analytical

Stable Water Isotope Analysis

Measurement of hydrogen and oxygen isotope ratios using Off-Axis Integrated Cavity Output Spectrometer with auto-sampler and heated injector block. Results reported in δ-notation relative to Vienna St...

0 papers