Protocols
2 protocols
samplingstandardized
Duchateau and Velthuis ovarian classification (Halictidae)
Collection of female halictid bees at nest sites using nets or vacuum devices, with individual storage and cryopreservation for chemical analysis. Includes morphological measurement and ovarian stage ...
analyticalstandardized
Cuticular hydrocarbon extraction and GC-FID analysis of sweat bees (Halictidae)
Extraction of cuticular compounds from individual bee specimens using n-pentane solvent followed by gas chromatographic analysis with flame ionization detection for chemical profile characterization. ...