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Protocols

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6 protocols

measurementstandardized

pavo visual modeling framework

Photography of butterfly landing sites using polarizing lens to measure landscape contrast between landing spots and surrounding areas. Photos analyzed using ImageJ to calculate gray scale contrast va...

optical4 paperscamera with polarizing lens, colored flags...
measurementstandardized

Drosophila courtship song recording via wing vibration (Drosophilidae)

Recording male Drosophila courtship songs by placing flies in Petri dish chambers and capturing wing vibration sounds with directional microphone positioned above the chamber roof.

acoustic recording3 papersPetri dish, nylon net...
measurement

Digital caliper morphometry of hummingbirds (Trochilidae)

Standard morphological measurements of hummingbird bill dimensions, mandible, and wing chord using digital calipers with duplicate measurements by different observers.

morphological measurement2 papershand calipers with digital display
laboratorystandardized

Isofemale line method (Drosophilidae)

Wild-caught flies are used to establish laboratory breeding lines maintained under standardized conditions. Lines are kept in multiple vials with controlled temperature, lighting, and nutrition to pre...

culture maintenance2 papersmalt medium, live yeast...
experimentalstandardized

Tetrachromatic color vision modeling (Trochilidae)

Novel method using programmable LED devices to test color discrimination in free-flying hummingbirds through reward-based choice experiments. Birds choose between tubes displaying different colors in ...

behavioral choice2 papersTetraColorTubes, tripod platforms...
sampling

mist net sampling (Trochilidae)

Hummingbirds are captured using a gravity-based drop-net system that releases around a suspended feeder onto a platform when triggered.

live capture2 papersbonnet trap, hummingbird feeder...