Protocols

Plant species composition within 15cm of Castilleja individuals is compared to random background vegetation to statistically identify overrepresented taxa as potential hosts. This provides an indirect...

Pressing and identification of plant voucher specimens using standard botanical keys and herbarium references.

Combined vertical (Robel pole) and horizontal (line intercept) measurements to quantify willow habitat structural complexity including height diversity, average height, patchiness, frequency and cover...

Calculation of Shannon diversity index (ln type) and Simpson dominance index (1-D) from species count data using online biodiversity calculator. Standard ecological diversity metrics for comparing com...

Systematic sampling of soil from different plant successional stages within a landslide area to compare soil properties across a chronosequence. Uses stratified random sampling within defined communit...

Complete census of sagebrush individuals within systematic 16m × 16m grids divided into 1m² cells, with measurement of plant dimensions for size estimation.

Conversion of reflectance spectra into bee and bird visual spaces using established photoreceptor sensitivity models. Uses color hexagon model for bees and receptor-noise limited model for birds to ca...

Long-term monitoring of tundra plant communities using standardized sampling within permanently marked study areas, employing various abundance quantification methods including point frame hits, visua...

Calculation of species area of occupancy (AOO) using filtered GBIF occurrence records and standardized grid-based methods following IUCN guidelines for conservation assessment.

Extraction of plant metabolites using methanol/acetonitrile/water solvent followed by liquid chromatography-mass spectrometry analysis. Characterizes plant metabolomic profiles for correlation with fu...

Comparative simulation study using five different soil organic carbon models (DAYCENT, CORPSE, MIMICS, MEND, RESOM) to project responses to warming and litter addition manipulations. Models were run u...

Systematic survey of pocket gopher soil disturbances (eskers, mounds) along standardized transects using rolatape measuring wheels with perpendicular PVC pipe to standardize observation width. Each di...

Using ArcGIS to digitize habitat polygons and generate random sampling points within appropriate community types.

Estimation of invertebrate biomass using established taxon-specific length-mass relationships and scaling to aerial measures.