Protocols

Belt transect method for censusing lupine populations and collecting inflorescences for morphological analysis across sympatric and allopatric sites. Combines population surveying with systematic flor...

A field-based protocol for extracting nectar from flowers that have been exposed to bee visitation, then quantifying ethanol content using enzyme-based assays. Flowers are pre-isolated to prevent nect...

Multi-temporal soil moisture measurements using TDR probe combined with soil coring and laboratory processing to determine moisture content, bulk density, and texture characteristics across environmen...

Field census method to quantify pollen receipt patterns in populations of gynodioecious plants with varying sex ratios and floral densities. Involves systematic counting of flowers by sex and measurem...

Moths were attracted using blacklight on white sheet, collected manually, and processed for pollen load analysis using fuchsin jelly swabs examined under microscopy. Network analysis characterized pla...

Uses centimeter-precision GPS to map exact locations of all individuals in study plots, enabling calculation of population density and neighbor relationships. Plot sizes are adjusted to population cha...

Method to quantify microbial load on different butterfly body parts by direct plating on agar media. Samples tarsi/proboscis by dragging across plate and thorax by washing technique.