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Refractometric nectar analysis
Local Knowledge Graph (41 entities)
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Description
A field-based protocol for extracting nectar from flowers that have been exposed to bee visitation, then quantifying ethanol content using enzyme-based assays. Flowers are pre-isolated to prevent nectar depletion before sampling.
Typical Equipment
- bags for flower isolation
- 10 μL microcapillary pipettes
- 0.6 mL microcentrifuge tubes
- qPCR-grade water
- parafilm
- freezer at -80°C
- balance for weighing
- handheld refractometer
- enzyme-based ethanol assay kit
- 96-well plates
- plate reader
Output Measurements
- nectar ethanol concentration
- total sugar concentration
- nectar mass
Papers Using This Protocol (27)
student_paper
Quantifying ethanol in bumble bee honey and Colorado rocky mountain wildflowers
student_paper
The effects of snowmelt date, soil moisture, and precipitation on nectar and floral morphology of Ipomopsis
student_paper
Temperature and relative humidity effects on nectar quantity, nectar quality, and plant-pollinator interactions
student_paper
Plasticity in Ipomopsis flower color and nectar production over space and time in response to water availability
student_paper
Effects of Experimental Water Addition on Floral Nectar Traits
student_paper
Effects of high temperature and early snowmelt on floral morphology and volatile organic compounds in <i> Ipomopsis aggregata </i>
student_paper
Effects of nectar robbing on the volatile organic compounds and nectar chemistry of intraindividual flowers in <i> Corydalis caseana </i> ssp. <i> brandegeei </i>
student_paper
Nectar Variation in Simple and Compound Flowers; and its Effects on Plant Pollinator Interactions
student_paper
Nectar Variation in Simple and Compound Flowers; and its Effects on Plant Pollinator Interactions
article
Competition for nectar resources does not affect bee foraging tactic constancy
student_paper
Quantifying Nectar Trait Responses to Natural Variation in Water Availability in Subalpine Plant Communities
student_paper
Impacts of snowmelt timing and precipitation on the expression of vegetative traits and floral traits in <i>Ipomopsis aggregata</i>
student_paper
Quantifying Nectar Resources in Bumble Bee Visited Plants
student_paper
Quantifying Nectar Resources in Bumble Bee Visited Plants
student_paper
Volatile organic compounds as signals for pollinators and their consistency across years
student_paper
Does road dust have an effect on nectar production in <i> pomopsis aggregata </i>?
student_paper
How does road dust influence Ipomopsis pollination?
student_paper
Characterizing nectar content in wet and dry subalpine meadows during an extreme summer drought
student_paper
Consequences of Nectar Robbing in Colorado Wildflowers: Insect Variation and Nectar Sugar Concentration
student_paper
Comparing clines in floral and vegetative traits along an elevation gradient in an <i>Ipomopsis</i> hybrid zone
article
Stimulation of flower nectar replenishment by removal: a survey of eleven animal-pollinated plant species
student_paper
The effects of climate change on plant traits and fruiting phenology of <i>Delphinium nuttallianum</i>.
student_paper
Nectar and pollen rewards of invasive plants and their native congeners
student_paper
Bumblebee foraging on vertical inflorescences: optimal or not?
student_paper
The effects of water and phosphorus on floral traits and nectar production of <i>Ipomopsis aggregata</i>
article
The dual role of floral traits: pollinator attraction and plant defense
article